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G3 (Bethesda)

Title:		The SR protein RSP-2 influences expression of the truncated insulin receptor DAF-2B in Caenorhabditis elegans
Author(s):		Martinez BA; Gill MS;
Address:		"Institute on the Biology of Aging and Metabolism and the Department of Genetics, Cell Biology and Development, University of Minnesota, 4-114 Nils Hasselmo Hall, 312 Church Street SE, Minneapolis, MN 55455, USA"
Journal Title:		G3 (Bethesda)
Year:		2023
Volume:		13
Issue:		6
Page Number:		-
DOI:		10.1093/g3journal/jkad064
ISSN/ISBN:		2160-1836 (Electronic) 2160-1836 (Linking)
Abstract:		"The alternatively spliced daf-2b transcript in Caenorhabditis elegans encodes a truncated isoform of the nematode insulin receptor that retains the extracellular ligand binding domain but lacks the intracellular signaling domain and is therefore unable to transduce a signal. To identify factors that influence expression of daf-2b, we performed a targeted RNA interference screen of rsp genes, which encode splicing factors from the serine/arginine protein family. Loss of rsp-2 significantly increased the expression of a fluorescent daf-2b splicing reporter, as well as increasing expression of endogenous daf-2b transcripts. Correspondingly, rsp-2 mutants exhibited similar phenotypes to those previously observed with DAF-2B overexpression, namely suppression of pheromone-induced dauer formation, enhancement of dauer entry in insulin signaling mutants, inhibition of dauer recovery, and increased lifespan. However, the epistatic relationship between rsp-2 and daf-2b varied according to the experimental context. Increased dauer entry and delayed dauer exit of rsp-2 mutants in an insulin signaling mutant background were partially dependent on daf-2b. Conversely, suppression of pheromone-induced dauer formation and increased lifespan in rsp-2 mutants were independent of daf-2b. These data demonstrate that C. elegans RSP-2, an ortholog of human splicing factor protein SRSF5/SRp40, is involved in regulating the expression of the truncated DAF-2B isoform. However, we also find that RSP-2 can influence dauer formation and lifespan independently of DAF-2B"
Keywords:		"Animals *Caenorhabditis elegans/metabolism *Caenorhabditis elegans Proteins/metabolism Insulin/metabolism Larva/genetics Mutation Pheromones/metabolism Protein Isoforms/genetics/metabolism Receptor, Insulin/genetics/metabolism aging dauer larva insulin ph;"
Notes:		"MedlineMartinez, Bryan A Gill, Matthew S eng P40 OD010440/OD/NIH HHS/ Research Support, N.I.H., Extramural England 2023/03/27 G3 (Bethesda). 2023 Jun 1; 13(6):jkad064. doi: 10.1093/g3journal/jkad064"