| Title: | "Overexpression of the homoterpene synthase gene, OsCYP92C21, increases emissions of volatiles mediating tritrophic interactions in rice" |
| Author(s): | Li W; Wang L; Zhou F; Li C; Ma W; Chen H; Wang G; Pickett JA; Zhou JJ; Lin Y; |
| Address: | "National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. School of Chemistry, Cardiff University, Cardiff, UK. Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, Lanzhou, China. College of Plant Protection, Gansu Agricultural University, Lanzhou, China" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Plant defence homoterpenes can be used to attract pest natural enemies. However, the biosynthetic pathway of homoterpenes is still unknown in rice, and the practical application of such indirect defence systems suffers from inherent limitations due to their low emissions from plants. Here, we demonstrated that the protein OsCYP92C21 is responsible for homoterpene biosynthesis in rice. We also revealed that the ability of rice to produce homoterpenes is dependent on the subcellular precursor pools. By increasing the precursor pools through specifically subcellular targeting expression, genetic transformation and genetic introgression, we significantly enhanced homoterpene biosynthesis in rice. The final introgressed GM rice plants exhibited higher homoterpene emissions than the wild type rice and the highest homoterpene emission reported so far for such GM plants even without the induction of herbivore attack. As a result, these GM rice plants demonstrated strong attractiveness to the parasitic wasp Cotesia chilonis. This study discovered the homoterpene biosynthesis pathway in rice, and lays the foundation for the utilisation of plant indirect defence mechanism in the 'push-pull' strategy of integrated pest management through increasing precursor pools in the subcellular compartments and overexpressing homoterpene synthase by genetic transformation" |
| Keywords: | "Alkyl and Aryl Transferases/*metabolism Animals CRISPR-Associated Protein 9 CRISPR-Cas Systems Gene Editing Gene Knockout Techniques Oryza/genetics/*metabolism *Plant Defense Against Herbivory Plant Proteins/*metabolism Plants, Genetically Modified Real-T;" |
| Notes: | "MedlineLi, Wei Wang, Lingnan Zhou, Fei Li, Changyan Ma, Weihua Chen, Hao Wang, Guirong Pickett, John A Zhou, Jing-Jiang Lin, Yongjun eng BB/J020281/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BB/L001683/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't 2020/10/26 Plant Cell Environ. 2021 Mar; 44(3):948-963. doi: 10.1111/pce.13924. Epub 2020 Dec 29" |
