| Title: | The Ycx1 protein encoded by the yeast YDL206W gene plays a role in calcium and calcineurin signaling |
| Author(s): | Lee GM; Weng F; Cranley J; Rajasekhar A; Stoeckel M; Kane T; Tisi R; Wang Y; |
| Address: | "Department of Biology, Saint Louis University, St Louis, Missouri, USA. Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milan, Italy. Department of Biology, Saint Louis University, St Louis, Missouri, USA. Electronic address: yuqi.wang@slu.edu" |
| DOI: | 10.1016/j.jbc.2023.104647 |
| ISSN/ISBN: | 1083-351X (Electronic) 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "Calcium is ubiquitously present in all living cells and plays important regulatory roles in a wide variety of biological processes. In yeast, many effects of calcium are mediated via the action of calcineurin, a calcium/calmodulin-dependent protein phosphatase. Proper signaling of calcium and calcineurin is important in yeast, and the calcineurin pathway has emerged as a valuable target for developing novel antifungal drugs. Here, we report a role of YDL206W in calcium and calcineurin signaling in yeast. YDL206W is an uncharacterized gene in yeast, encoding a protein with two sodium/calcium exchange domains. Disrupting the YDL206W gene leads to a diminished level of calcium-induced activation of calcineurin and a reduced accumulation of cytosolic calcium. Consistent with a role of calcineurin in regulating pheromone and cell wall integrity signaling, the ydl206wDelta mutants display an enhanced growth arrest induced by pheromone treatment and poor growth at elevated temperature. Subcellular localization studies indicate that YDL206W is localized in endoplasmic reticulum and Golgi. Together, our results reveal YDL206W as a new regulator for calcineurin signaling in yeast and suggest a role of the endoplasmic reticulum and Golgi in regulating cytosolic calcium in yeast" |
| Keywords: | "Calcineurin/genetics/metabolism Calcium/metabolism *Saccharomyces cerevisiae/metabolism *Saccharomyces cerevisiae Proteins/genetics/metabolism *Signal Transduction/genetics Gene Expression Regulation, Fungal/genetics Chitin/metabolism Ydl206w Ycx1 calcine;" |
| Notes: | "MedlineLee, Grace M Weng, Fangli Cranley, Juliana Rajasekhar, Abhinav Stoeckel, Matthew Kane, Thomas Tisi, Renata Wang, Yuqi eng R15 GM141652/GM/NIGMS NIH HHS/ R15 GM106330/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2023/03/26 J Biol Chem. 2023 May; 299(5):104647. doi: 10.1016/j.jbc.2023.104647. Epub 2023 Mar 24" |
