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Glycobiology

Title:		"Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked beta-1,3-/beta-1,6-glucan heteropolymer"
Author(s):		Kapteyn JC; Montijn RC; Vink E; de la Cruz J; Llobell A; Douwes JE; Shimoi H; Lipke PN; Klis FM;
Address:		"Institute of Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The Netherlands"
Journal Title:		Glycobiology
Year:		1996
Volume:		6
Issue:		3
Page Number:		337 - 345
DOI:		10.1093/glycob/6.3.337
ISSN/ISBN:		0959-6658 (Print) 0959-6658 (Linking)
Abstract:		"Yeast cell wall proteins, including Cwp1p and alpha-agglutinin, could be released by treating the cell wall with either beta-1,3-or beta-1,6-glucanases, indicating that both polymers are involved in anchoring cell wall proteins. It was shown immunologically that both beta-1,3- and beta-1,6-glucan were linked to yeast cell wall proteins, including Cwp1p and alpha-agglutinin. It was further shown that beta-1,3-glucan was linked to the wall protein through a beta-1,6-glucan moiety. The beta-1,6-glucan moiety could be removed from Cwp1p and other cell wall proteins by cleaving phosphodiester bridges either enzymatically using phosphodiesterases or chemically using ice-cold aqueous hydrofluoric acid. These observations are consistent with the notion that cell wall proteins in Saccharomyces cerevisiae are linked to a beta-1,3-/beta-1,6-glucan heteropolymer through a phosphodiester linkage and that this polymer is responsible for anchoring cell wall proteins. It is proposed that this polymer is identical to the alkali-soluble beta-1,3-/beta-1,6-glucan heteropolymer characterized by Fleet and Manners (1976, 1977)"
Keywords:		"Biopolymers/chemistry/metabolism Carbohydrate Conformation Cell Wall/chemistry/*metabolism Fungal Proteins/*metabolism Glucan 1, 3-beta-Glucosidase Glucans/chemistry/*metabolism Glycoside Hydrolases Mating Factor Membrane Glycoproteins/chemistry/metabolism;"
Notes:		"MedlineKapteyn, J C Montijn, R C Vink, E de la Cruz, J Llobell, A Douwes, J E Shimoi, H Lipke, P N Klis, F M eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1996/04/01 Glycobiology. 1996 Apr; 6(3):337-45. doi: 10.1093/glycob/6.3.337"