| Title: | Sequence-directed bends of DNA helix axis at the upstream activation sites of alpha-cell-specific genes in yeast |
| Author(s): | Inokuchi K; Nakayama A; Hishinuma F; |
| Address: | "Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan" |
| ISSN/ISBN: | 0305-1048 (Print) 1362-4962 (Electronic) 0305-1048 (Linking) |
| Abstract: | "The MF alpha 1 gene of Saccharomyces cerevisiae, a major structural gene for mating pheromone alpha factor, is an alpha-cell-specific gene whose expression is regulated by the mating-type locus, MAT. Two upstream activation sites (UASMF alpha 1s), which are binding sites for an activator protein, MAT alpha 1, mediate alpha-cell-specific expression of this gene. We show here that DNA fragments containing the UASMF alpha 1 region exhibited anomalous slow electrophoretic mobilities on gels at lower temperature, but not at higher temperature, that is characteristic of bent DNA. We confirmed the sequence-directed bend at the UASMF alpha 1 region by employing a circular permutation analysis and a DNA cyclization assay. Deletion analyses revealed the existence of two bends in this region, each of which overlaps each UASMF alpha 1 element. The two bends were almost in phase; they lie in a nearly same plane with a same direction. We also show the existence of sequence-directed bend at the UAS region of the STE3 gene, another alpha-specific gene in S. cerevisiae. These bent DNAs may be involved in transcriptional regulation of this set of genes" |
| Keywords: | "Base Sequence DNA Mutational Analysis DNA, Fungal/*genetics *Genes, Fungal *Genes, Mating Type, Fungal Mating Factor Molecular Sequence Data Nucleic Acid Conformation Peptides/*genetics *Promoter Regions, Genetic *Regulatory Sequences, Nucleic Acid Saccha;" |
| Notes: | "MedlineInokuchi, K Nakayama, A Hishinuma, F eng Research Support, Non-U.S. Gov't England 1988/07/25 Nucleic Acids Res. 1988 Jul 25; 16(14B):6693-711. doi: 10.1093/nar/16.14.6693" |
