| Title: | SARS CoV-2 infection screening via the exhaled breath fingerprint obtained by FTIR spectroscopic gas-phase analysis. A proof of concept |
| Author(s): | Glockler J; Mizaikoff B; Diaz de Leon-Martinez L; |
| Address: | "Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany. Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany; Hahn-Schickard Institute for Microanalysis Systems, Sedanstrasse 14, 89077 Ulm, Germany. Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany. Electronic address: lorena.diaz-de-leon@uni-ulm.de" |
| Journal Title: | Spectrochim Acta A Mol Biomol Spectrosc |
| DOI: | 10.1016/j.saa.2023.123066 |
| ISSN/ISBN: | 1873-3557 (Electronic) 1386-1425 (Print) 1386-1425 (Linking) |
| Abstract: | "The COVID-19 pandemic remains a global challenge now with the long-COVID arising. Mitigation measures focused on case counting, assessment and determination of variants and their likely targets of infection and transmission, the pursuit of drug treatments, use and enhancement of masks, social distancing, vaccination, post-infection rehabilitation, and mass screening. The latter is of utmost importance given the current scenario of infections, reinfections, and long-term health effects. Research on screening platforms has been developed to provide more sensitive, specific, and reliable tests that are accessible to the entire population and can be used to assess the prognosis of the disease as well as the subsequent health follow-up of patients with sequelae of COVID-19. Therefore, the aim of the present study was the simulation of exhaled breath of COVID-19 patients by evaluation of three identified COVID-19 indicator breath biomarkers (acetone (ACE), acetaldehyde (ACH) and nitric oxide (NO)) by gas-phase infrared spectroscopy as a proof-of-concept principle for the detection of infected patients' exhaled breath fingerprint and subsequent follow-up. The specific fingerprints of each of the compounds and the overall fingerprint were obtained. The synthetic exhaled breath evaluation concept revealed a linearity of r = 0.99 for all compounds, and LODs of 6.42, 13.81, 9.22 ppm, and LOQs of 42.26, 52.57, 69.23 ppm for NO, ACE, and ACH, respectively. This study proves the fundamental feasibility of gas-phase infrared spectroscopy for fingerprinting lung damage biomarkers in exhaled breath of patients with COVID-19. This analysis would allow faster and cheaper screening and follow-up of infected individuals, which could improve mass screening in POC settings" |
| Keywords: | "Humans Spectroscopy, Fourier Transform Infrared Post-Acute COVID-19 Syndrome Pandemics Breath Tests/methods *covid-19 Nitric Oxide Acetaldehyde Biomarkers *Volatile Organic Compounds/analysis Covid-19 Exhaled breath Gas-phase infrared spectroscopy Screeni;" |
| Notes: | "MedlineGlockler, Johannes Mizaikoff, Boris Diaz de Leon-Martinez, Lorena eng England 2023/06/26 Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5; 302:123066. doi: 10.1016/j.saa.2023.123066. Epub 2023 Jun 22" |
