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Biochim Biophys Acta

Title:		Studies on the receptors to 5alpha-androst-16-en-3-one and 5alpha-androst-16-en-3alpha-ol in sow nasal mucosa
Author(s):		Gennings JN; Gower DB; Bannister LH;
Address:
Journal Title:		Biochim Biophys Acta
Year:		1977
Volume:		496
Issue:		2
Page Number:		547 - 556
DOI:		10.1016/0304-4165(77)90335-x
ISSN/ISBN:		0006-3002 (Print) 0006-3002 (Linking)
Abstract:		"The presence of receptors to the 'boar taint' pheromones 5alpha-androst-16-en-3-one and 5alpha-androst-16-en-3alpha-ol has been demonstrated in sow olfactory mucosa. Binding studies indicated that a sufficiently low concentration of olfactory tissue homogenate exhibited saturation of binding of 5alpha-androst-16-en-3-one, and this was of high affinity compared with control tissues of non-olfactory and heated olfactory tissues. Analysis of receptor binding of 5alpha-androst-16-en-3-one gave a value for the affinity constant (Ka) of approx. 8.3-10(8) M-1 and the value for the molar concentration of binding sites (n[M]) was approx. 3.3 pmol/mg protein. Almost identical values of Ka and n [M] were obtained when receptor binding of 5alpha-[5alpha-3H]androst-16-en-3alpha-ol was investigated (Ka 8.4-10(8) M-1; n [M] 3.7 pmol/mg protein). This suggests that the same receptor binds both 5alpha-androst-16-en-3-one and 5alpha-androst-16-en-3alpha-ol with equally high affinity. In a preliminary investigation to establish the specificity of the receptor, the binding of 17beta-hydroxy-5alpha-androstan-3-one was assayed; this steroid is odourless but has a similar structure except in ring D to 5alpha-androst-16-en-3-one. Binding was of the low affinity, non-specific type only, indicating that the sow olfactory receptors are not sensitive to this androgen"
Keywords:		"Androstenes/*metabolism Androstenols/*metabolism Animals Dihydrotestosterone/metabolism Female Nasal Mucosa/*metabolism Pheromones/*metabolism Receptors, Steroid/*metabolism Swine Temperature;"
Notes:		"MedlineGennings, J N Gower, D B Bannister, L H eng Netherlands 1977/02/28 Biochim Biophys Acta. 1977 Feb 28; 496(2):547-56. doi: 10.1016/0304-4165(77)90335-x"