| Title: | Isolation and expression of cytochrome P450 genes in the antennae and gut of pine beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) following exposure to host monoterpenes |
| Author(s): | Cano-Ramirez C; Lopez MF; Cesar-Ayala AK; Pineda-Martinez V; Sullivan BT; Zuniga G; |
| Address: | "Departamento de Zoologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala, Col. Sto. Tomas, Mexico D. F. CP 11340, Mexico" |
| DOI: | 10.1016/j.gene.2012.11.059 |
| ISSN/ISBN: | 1879-0038 (Electronic) 0378-1119 (Linking) |
| Abstract: | "Bark beetles oxidize the defensive monoterpenes of their host trees both to detoxify them and convert them into components of their pheromone system. This oxidation is catalyzed by cytochrome P450 enzymes and occurs in different tissues of the insect, including the gut (i.e., the site where the beetle's pheromones are produced and accumulated) and the antennae (i.e., the olfactory organs used for perception of airborne defensive monoterpenes as well as other host-associated compounds and pheromones). We identified ten new CYP genes in the pine beetle Dendroctonus rhizophagus in either antennae or gut tissue after stimulation with the vapors of major host monoterpenes alpha-pinene, beta-pinene and 3-carene. Five genes belong to the CYP4 family, four to the CYP6 family and one to the CYP9 family. Differential expression of almost all of the CYP genes was observed between sexes, and within these significant differences among time, stimuli, anatomical region, and their interactions were found upon exposure to host monoterpenes. Increased expression of cytochrome P450 genes suggests that they play a role in the detoxification of monoterpenes released by this insect's host trees" |
| Keywords: | "Animals Arthropod Antennae/metabolism Base Sequence Cytochrome P-450 Enzyme System/*genetics/metabolism Female Gene Expression Regulation/*drug effects *Genes, Insect Intestinal Mucosa/metabolism Male Molecular Sequence Data Monoterpenes/*pharmacology Mul;" |
| Notes: | "MedlineCano-Ramirez, Claudia Lopez, Maria Fernanda Cesar-Ayala, Ana K Pineda-Martinez, Veronica Sullivan, Brian T Zuniga, Gerardo eng Research Support, Non-U.S. Gov't Netherlands 2012/12/25 Gene. 2013 May 10; 520(1):47-63. doi: 10.1016/j.gene.2012.11.059. Epub 2012 Dec 20" |
