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Sensors (Basel)

Title:		A Pilot Study for Legionella pneumophila Volatilome Characterization Using a Gas Sensor Array and GC/MS Techniques
Author(s):		Capuano R; Mansi A; Paba E; Marcelloni AM; Chiominto A; Proietto AR; Gordiani A; Catini A; Paolesse R; Tranfo G; Di Natale C;
Address:		"Department of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, Italy. Interdepartmental Centre for Volatilomics 'A. D'Amico', University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, Italy. Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers' Compensation Authority (INAIL), Via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy. Department of Chemical Science and Technology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy"
Journal Title:		Sensors (Basel)
Year:		2023
Volume:		20230126
Issue:		3
Page Number:		-
DOI:		10.3390/s23031401
ISSN/ISBN:		1424-8220 (Electronic) 1424-8220 (Linking)
Abstract:		"Legionellosis is a generic term describing the pneumonic (Legionnaires' disease, LD) and non-pneumonic (Pontiac fever, PF) forms of infection with bacteria belonging to the genus Legionella. Currently, the techniques used to detect Legionella spp. in water samples have certain limitations and drawbacks, and thus, there is a need to identify new tools to carry out low-cost and rapid analysis. In this regard, several studies demonstrated that a volatolomics approach rapidly detects and discriminates different species of microorganisms via their volatile signature. In this paper, the volatile organic compounds (VOCs) pattern emitted in vitro by Legionella pneumophila cultures is characterized and compared to those produced by other Legionella species and by Pseudomonas aeruginosa, using a gas sensor array and gas chromatograph mass spectrometer (GC-MS). Bacterial cultures were measured at the 3rd and 7th day after the incubation. Sensor array data analyzed via the K-nearest neighbours (k-NN) algorithm showed a sensitivity to Legionella pneumophila identification at around 89%. On the other hand, GC-MS identified a bouquet of VOCs, mainly alcohols and ketones, that enable the differentiation of Legionella pneumophila in respect to other waterborne microorganisms"
Keywords:		Humans *Legionella pneumophila Pilot Projects Gas Chromatography-Mass Spectrometry *Legionnaires' Disease/diagnosis/microbiology *Legionella Legionella pneumophila gas sensor array volatile organic compounds volatilome;
Notes:		"MedlineCapuano, Rosamaria Mansi, Antonella Paba, Emilia Marcelloni, Anna Maria Chiominto, Alessandra Proietto, Anna Rita Gordiani, Andrea Catini, Alexandro Paolesse, Roberto Tranfo, Giovanna Di Natale, Corrado eng BRIC 2019-ID07/Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro/ Switzerland 2023/02/12 Sensors (Basel). 2023 Jan 26; 23(3):1401. doi: 10.3390/s23031401"