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Pestic Biochem Physiol

Title:		A Delta9 desaturase (SlitDes11) is associated with the biosynthesis of ester sex pheromone components in Spodoptera litura
Author(s):		Zhang YN; Zhang XQ; Zhu GH; Zheng MY; Yan Q; Zhu XY; Xu JW; Zhang YY; He P; Sun L; Palli SR; Zhang LW; Dong SL;
Address:		"College of Life Sciences, Huaibei Normal University, Huaibei, China. Electronic address: ynzhang_insect@163.com. Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China; Department of Entomology, University of Kentucky, Lexington, USA. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. College of Life Sciences, Huaibei Normal University, Huaibei, China. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China. Department of Entomology, University of Kentucky, Lexington, USA. Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China. Electronic address: zhanglw@ahau.edu.cn. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Electronic address: sldong@njau.edu.cn"
Journal Title:		Pestic Biochem Physiol
Year:		2019
Volume:		20190227
Issue:
Page Number:		152 - 159
DOI:		10.1016/j.pestbp.2019.02.018
ISSN/ISBN:		1095-9939 (Electronic) 0048-3575 (Linking)
Abstract:		"Sex pheromone biosynthesis in moths relies on the activity of multiple enzymes, including Delta9 desaturase, which plays an important role in catalyzing desaturation at the Delta9 position of the carbon chain. However, the physiological function of moth Delta9 desaturase has not been elucidated in vivo. In this study, we used the CRISPR/Cas9 system to knockout the Delta9 desaturase gene (SlitDes11) of Spodoptera litura to analyze its role in sex pheromone biosynthesis. First, through the direct injection of SlitDes11-single guide RNA (sgRNA)/Cas9 messenger RNA into newly laid eggs, gene editing was induced in around 30% of eggs 24?ª+h after injection and was induced in 20.8% of the resulting adult moths. Second, using a sibling-crossing strategy, insects with mutant SlitDes11 (bearing a premature stop codon) were selected, and homozygous mutants were obtained in the G5 generation. Third, pheromone gland extracts of adult female homozygous SlitDes11 mutants were analyzed using Gas chromatography (GC). The results showed that titers of all three ester sex pheromone components; Z9, E11-14:Ac, Z9,E12-14:Ac, and Z9-14:Ac; were reduced by 62.40%, 78.50%, and 72.50%, respectively. This study provides the first direct evidence for the role of SlitDes11 in sex pheromone biosynthesis in S. litura, and indicates the gene could be as potential target to disrupt sexual communication in S. litura for developing a new pollution-free insecticide"
Keywords:		"Amino Acid Sequence Animals Female Germ-Line Mutation Insect Proteins/chemistry/genetics/*metabolism Moths/metabolism Mutation/genetics RNA, Messenger Sequence Alignment Sex Attractants/*metabolism Spodoptera/*metabolism Stearoyl-CoA Desaturase/*metabolis;"
Notes:		"MedlineZhang, Ya-Nan Zhang, Xiao-Qing Zhu, Guan-Heng Zheng, Mei-Yan Yan, Qi Zhu, Xiu-Yun Xu, Ji-Wei Zhang, Yun-Ying He, Peng Sun, Liang Palli, Subba Reddy Zhang, Long-Wa Dong, Shuang-Lin eng 2019/04/28 Pestic Biochem Physiol. 2019 May; 156:152-159. doi: 10.1016/j.pestbp.2019.02.018. Epub 2019 Feb 27"