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J Neurosci

Title:		Molecular genetic analysis of sexual rejection: roles of octopamine and its receptor OAMB in Drosophila courtship conditioning
Author(s):		Zhou C; Huang H; Kim SM; Lin H; Meng X; Han KA; Chiang AS; Wang JW; Jiao R; Rao Y;
Address:		"Institute of Biophysics and the Graduate School of the Chinese Academy of Sciences, Beijing 100101, People's Republic of China"
Journal Title:		J Neurosci
Year:		2012
Volume:		32
Issue:		41
Page Number:		14281 - 14287
DOI:		10.1523/JNEUROSCI.0517-12.2012
ISSN/ISBN:		1529-2401 (Electronic) 0270-6474 (Print) 0270-6474 (Linking)
Abstract:		"After Drosophila males are rejected by mated females, their subsequent courtship is inhibited even when encountering virgin females. Molecular mechanisms underlying courtship conditioning in the CNS are unclear. In this study, we find that tyramine beta hydroxylase (TbetaH) mutant males unable to synthesize octopamine (OA) showed impaired courtship conditioning, which could be rescued by transgenic TbetaH expression in the CNS. Inactivation of octopaminergic neurons mimicked the TbetaH mutant phenotype. Transient activation of octopaminergic neurons in males not only decreased their courtship of virgin females, but also produced courtship conditioning. Single cell analysis revealed projection of octopaminergic neurons to the mushroom bodies. Deletion of the OAMB gene encoding an OA receptor expressed in the mushroom bodies disrupted courtship conditioning. Inactivation of neurons expressing OAMB also eliminated courtship conditioning. OAMB neurons responded robustly to male-specific pheromone cis-vaccenyl acetate in a dose-dependent manner. Our results indicate that OA plays an important role in courtship conditioning through its OAMB receptor expressed in a specific neuronal subset of the mushroom bodies"
Keywords:		"Animals *Conditioning, Psychological/physiology *Courtship Drosophila Proteins/*genetics/physiology Drosophila melanogaster Female *Gene Knock-In Techniques/methods Male Octopamine/*genetics/physiology Receptors, Neurotransmitter/*genetics/physiology Sexu;"
Notes:		"MedlineZhou, Chuan Huang, Hai Kim, Susy M Lin, Hsuanwen Meng, Xianglan Han, Kyung-An Chiang, Ann-Shyn Wang, Jing W Jiao, Renjie Rao, Yi eng R01 DC009597/DC/NIDCD NIH HHS/ R01 DK092640/DK/NIDDK NIH HHS/ Research Support, Non-U.S. Gov't 2012/10/12 J Neurosci. 2012 Oct 10; 32(41):14281-7. doi: 10.1523/JNEUROSCI.0517-12.2012"