| Title: | Both Allene Oxide Synthases Genes Are Involved in the Biosynthesis of Herbivore-Induced Jasmonic Acid and Herbivore Resistance in Rice |
| Author(s): | Zeng J; Zhang T; Huangfu J; Li R; Lou Y; |
| Address: | "State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. College of Food Science, Southwest University, Chongqing 400715, China" |
| ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
| Abstract: | "Allene oxide synthase (AOS) is the second enzyme in the biosynthesis of the plant defensive hormone jasmonic acid (JA). In rice, there are two AOSs, OsAOS1 and OsAOS2. However, the role of these two AOS genes in herbivore-induced defenses in rice remains unidentified. We cloned the two rice AOS genes and observed that the transcript level of both OsAOS1 and OsAOS2 was enhanced by mechanical wounding, the infestation of the striped stem borer (SSB) (Chilo suppressalis) or brown planthopper (BPH) (Niaparvata lugens), and treatment with JA; however, OsAOS1 responded more rapidly to SSB infestation and JA treatment than did OsAOS2. The antisense expression of OsAOS1 (as-aos1) or OsAOS2 (as-aos2) decreased levels of SSB- or BPH-induced JA, which, in turn, reduced the production of SSB-induced trypsin protease inhibitor (TrypPI) and volatiles as well as the resistance of rice to SSB. In contrast, BPH preferred to feed and oviposit on wild-type (WT) plants over as-aos1 and as-aos2 plants. Moreover, the survival of BPH nymphs on as-aos1 or as-aos2 lines was significantly lower than on WT plants. The increased resistance of as-aos1 or as-aos2 plants to BPH correlated with higher levels of BPH-induced H(2)O(2) and SA. These results indicate that OsAOS1 and OsAOS2 are both involved in herbivore-induced JA biosynthesis and play a vital role in determining the resistance of rice to chewing and phloem-feeding herbivores" |
| Keywords: | allene oxide synthase herbivore resistance hydrogen peroxide jasmonic acid rice salicylic acid; |
| Notes: | "PubMed-not-MEDLINEZeng, Jiamei Zhang, Tongfang Huangfu, Jiayi Li, Ran Lou, Yonggen eng 31930091/National Natural Science Foundation of China/ Switzerland 2021/03/04 Plants (Basel). 2021 Feb 26; 10(3):442. doi: 10.3390/plants10030442" |
