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Microbiol Res

Title:		Antifungal mechanisms of volatile organic compounds produced by Pseudomonas fluorescens ZX as biological fumigants against Botrytis cinerea
Author(s):		Yue Y; Wang Z; Zhong T; Guo M; Huang L; Yang L; Kan J; Zalan Z; Hegyi F; Takacs K; Du M;
Address:		"College of Food Science, Southwest University, 2# Tiansheng Road, Beibei, Chongqing 400715, PR China; Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, PR China. College of Food Science, Southwest University, 2# Tiansheng Road, Beibei, Chongqing 400715, PR China; Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, PR China. Electronic address: wangzhirongcn@163.com. College of Food Science, Southwest University, 2# Tiansheng Road, Beibei, Chongqing 400715, PR China; Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, PR China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, PR China. Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, PR China; Food Science and Technology Institute, Hungarian University of Agriculture and Life Sciences, Buda Campus, 1022, Herman Otto str. 15, Hungary. College of Food Science, Southwest University, 2# Tiansheng Road, Beibei, Chongqing 400715, PR China; Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, PR China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, PR China. Electronic address: muyingdu_swu@163.com"
Journal Title:		Microbiol Res
Year:		2023
Volume:		20221113
Issue:
Page Number:		127253 -
DOI:		10.1016/j.micres.2022.127253
ISSN/ISBN:		1618-0623 (Electronic) 0944-5013 (Linking)
Abstract:		"To explore the antifungal mechanisms of volatile organic compounds (VOCs) produced by Pseudomonas fluorescens ZX against Botrytis cinerea, biochemical analyses and transcriptomic techniques were employed in this work. The results showed that P. fluorescens ZX-producing VOCs can increase the cell membrane permeability of B. cinerea and disrupt cell membrane integrity, resulting in leakage of the pathogen's cellular contents, inhibition of ergosterol biosynthesis (about 76%), and an increase in malondialdehyde (MDA) content. Additionally, for B. cinerea respiration, P. fluorescens ZX-producing VOCs (1 x 10(9) CFU /mL) significantly inhibited the activities of ATPase (55.7%), malate dehydrogenase (MDH) (33.1%), and succinate dehydrogenase (SDH) (57.9%), seriously interfering with energy metabolism and causing accumulation of reactive oxygen species (ROS). Furthermore, transcriptome analysis of B. cinerea following exposure to VOCs revealed 4590 differentially expressed genes (DEGs) (1388 upregulated, 3202 downregulated). Through GO analysis, these DEGs were determined to be enriched in intrinsic components of membrane, integral components of membrane, and membrane parts, while KEGG analysis indicated that they were enriched in many amino acid metabolism pathways. Significantly, the DEGs related to ergosterol biosynthesis, ATPase, mitochondrial respiratory chain, malate dehydrogenase, and cell membrane showed down-regulation, corroborating the biochemical analyses. Taken together, these results suggest that the antifungal activity of P. fluorescens ZX-producing VOCs against B. cinerea occurs primary mechanisms: causing significant damage to the cell membrane, negatively affecting respiration, and interfering with amino acid metabolism"
Keywords:		Adenosine Triphosphatases/metabolism Amino Acids/metabolism *Antifungal Agents/chemistry/metabolism Botrytis Ergosterol/metabolism Malate Dehydrogenase/metabolism Plant Diseases/microbiology *Pseudomonas fluorescens/chemistry/metabolism *Volatile Organic;
Notes:		"MedlineYue, Yusen Wang, Zhirong Zhong, Tao Guo, Meiling Huang, Luhan Yang, Lili Kan, Jianquan Zalan, Zsolt Hegyi, Ferenc Takacs, Krisztina Du, Muying eng Germany 2022/12/02 Microbiol Res. 2023 Feb; 267:127253. doi: 10.1016/j.micres.2022.127253. Epub 2022 Nov 13"