| Title: | Molecular evidence of RNA editing in Bombyx chemosensory protein family |
| Author(s): | Xuan N; Bu X; Liu YY; Yang X; Liu GX; Fan ZX; Bi YP; Yang LQ; Lou QN; Rajashekar B; Leppik G; Kasvandik S; Picimbon JF; |
| Address: | "Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China. Shandong Silkworm Institute, Shandong Academy of Agricultural Sciences, Yantai, Shandong Province, China. Institute of Computer Science, University of Tartu, Tartu, Tartumaa Province, Estonia. Proteomics Core Facility, Institute of Technology, University of Tartu, Tartu, Tartumaa Province, Estonia" |
| DOI: | 10.1371/journal.pone.0086932 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Chemosensory proteins (CSPs) are small scavenger proteins that are mainly known as transporters of pheromone/odor molecules at the periphery of sensory neurons in the insect antennae and in the producing cells from the moth female pheromone gland. Sequencing cDNAs of RNA encoding CSPs in the antennae, legs, head, pheromone gland and wings from five single individual adult females of the silkworm moth Bombyx mori showed that they differed from genomic sequences by subtle nucleotide replacement (RDD). Both intronless and intronic CSP genes expressed RDDs, although in different rates. Most interestingly, in our study the degree of RDDs in CSP genes were found to be tissue-specific. The proportion of CSP-RDDs was found to be significantly much higher in the pheromone gland. In addition, Western blot analysis of proteins in different tissues showed existence of multiple CSP protein variant chains particularly found in the pheromone gland. Peptide sequencing demonstrated the occurrence of a pleiad of protein variants for most of all BmorCSPs from the pheromone gland. Our findings show that RNA editing is an important feature in the expression of CSPs and that a high variety of RDDs is found to expand drastically thus altering the repertoire of CSP proteins in a tissue-specific manner" |
| Keywords: | "Amino Acid Sequence Animals Arthropod Antennae/metabolism Base Sequence Bombyx/*genetics/metabolism DNA, Complementary Female *Genome, Insect Insect Proteins/*genetics/metabolism Molecular Sequence Data Organ Specificity Pheromones/*genetics/metabolism Po;" |
| Notes: | "MedlineXuan, Ning Bu, Xun Liu, Yan Yan Yang, Xue Liu, Guo Xia Fan, Zhong Xue Bi, Yu Ping Yang, Lian Qun Lou, Qi Nian Rajashekar, Balaji Leppik, Getter Kasvandik, Sergo Picimbon, Jean-Francois eng Research Support, Non-U.S. Gov't 2014/02/20 PLoS One. 2014 Feb 13; 9(2):e86932. doi: 10.1371/journal.pone.0086932. eCollection 2014" |
