| Title: | Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating |
| Author(s): | Wu L; van Peer A; Song W; Wang H; Chen M; Tan Q; Song C; Zhang M; Bao D; |
| Address: | "Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P.R. China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P.R. China; National Engineering Research Center of Edible Fungi, Ministry of Science and Technology, P.R. China" |
| DOI: | 10.1016/j.gene.2013.08.090 |
| ISSN/ISBN: | 1879-0038 (Electronic) 0378-1119 (Linking) |
| Abstract: | "During the life cycle of heterothallic tetrapolar Agaricomycetes such as Lentinula edodes (Berk.) Pegler, the mating type system, composed of unlinked A and B loci, plays a vital role in controlling sexual development and resulting formation of the fruit body. L. edodes is produced worldwide for consumption and medicinal purposes, and understanding its sexual development is therefore of great importance. A considerable amount of mating type factors has been indicated over the past decades but few genes have actually been identified, and no complete genetic structures of L. edodes B mating-type loci are available. In this study, we cloned the matB regions from two mating compatible L. edodes strains, 939P26 and 939P42. Four pheromone receptors were identified on each new matB region, together with three and four pheromone precursor genes in the respective strains. Gene polymorphism, phylogenetic analysis and distribution of pheromone receptors and pheromone precursors clearly indicate a bipartite matB locus, each sublocus containing a pheromone receptor and one or two pheromone precursors. Detailed sequence comparisons of genetic structures between the matB regions of strains 939P42, 939P26 and a previously reported strain SUP2 further supported this model and allowed identification of the B mating type subloci borders. Mating studies confirmed the control of B mating by the identified pheromone receptors and pheromones in L. edodes" |
| Keywords: | "Amino Acid Sequence Cloning, Molecular Gene Expression Regulation, Fungal/genetics Genes, Mating Type, Fungal/*genetics Genetic Loci/*genetics Molecular Sequence Data Organisms, Genetically Modified Phylogeny *Regulatory Sequences, Nucleic Acid/physiology;" |
| Notes: | "MedlineWu, Lin van Peer, Arend Song, Wenhua Wang, Hong Chen, Mingjie Tan, Qi Song, Chunyan Zhang, Meiyan Bao, Dapeng eng Research Support, Non-U.S. Gov't Netherlands 2013/09/14 Gene. 2013 Dec 1; 531(2):270-8. doi: 10.1016/j.gene.2013.08.090. Epub 2013 Sep 9" |
