Title: | Identification of new sex pheromone plasmids in Enterococcus faecalis |
Author(s): | Wirth R; Friesenegger A; Horaud T; |
Address: | "Lehrstuhl fur Mikrobiologie, Universitat Munchen, FRG" |
ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
Abstract: | "We describe the identification of the following new sex pheromone plasmids in Enterococcus faecalis: a haemolysin-bacteriocin plasmid, pIP964; three R plasmids, pIP1017, pIP1438 and pIP1440; and two cryptic conjugative plasmids, pIP1141 and pMV120. The identification was based on the formation of cell aggregates on filter membranes during conjugation, on efficient transfer in broth matings, and on a positive clumping reaction of cells carrying these plasmids. In addition these plasmids hybridized with DNA probes specific for sex pheromone-induced structural genes encoding surface proteins required for conjugative transfer of the plasmids" |
Keywords: | "DNA Probes DNA, Bacterial/genetics Enterococcus faecalis/*genetics Genes, Bacterial Nucleic Acid Hybridization Open Reading Frames *Plasmids Sex Attractants/*genetics;" |
Notes: | "MedlineWirth, R Friesenegger, A Horaud, T eng Research Support, Non-U.S. Gov't Germany 1992/05/01 Mol Gen Genet. 1992 May; 233(1-2):157-60. doi: 10.1007/BF00587574" |