| Title: | The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
| Author(s): | Wanderley LF; Batista KLR; Carvalho JF; Lima ADS; Landulfo GA; Soares A; Costa LMJ; |
| Address: | "Curso de Engenharia Quimica, Universidade Federal do Maranhao - UFMA, Sao Luis, MA, Brasil. Departamento de Patologia, Universidade Federal do Maranhao - UFMA, Sao Luis, MA, Brasil" |
| DOI: | 10.1590/S1984-29612017026 |
| ISSN/ISBN: | 1984-2961 (Electronic) 0103-846X (Linking) |
| Abstract: | "Plants respond to wounding caused by mechanical stress or herbivory by synthesizing defense proteins. There are no studies reporting the action of induced plant proteins against ticks. The aim of this study was to investigate the effect of mechanically wounded Leucaena leucocephala leaves against Rhipicephalus (Boophilus) microplus. Initially, we carried out time course experiments to evaluate the impact of mechanical wounding on the protein content and the peroxidase, catalase and protease inhibitor activities in L. leucocephala. We then evaluated the acaricidal activity on R. (B.) microplus from protein extract collected from L. leucocephala after mechanical wounding. L. leucocephala leaves were artificially wounded, and after 6, 12, 24 and 48h, the leaves were collected for protein extraction. Quantitative and qualitative analyses of the proteins were performed. The protein content and peroxidase and protease activities increased 12h after wounding, and the acaricidal activity of this protein extract was evaluated using engorged R. (B.) microplus females. The protein extract obtained after wounding reduced egg production (8.5%) compared to those without wounding. Furthermore, the extract reduced egg hatching by 47.7% and showed an overall efficacy of 56.3% at 0.1 mgP/mL of the protein. We demonstrated that L. leucocephala defensive proteins could be effective against R. (B.) microplus" |
| Keywords: | "Acaricides/metabolism/*pharmacology Animals Fabaceae/*metabolism Female Larva/drug effects Plant Extracts/*pharmacology Plant Leaves/*metabolism Rhipicephalus/*drug effects *Stress, Mechanical;" |
| Notes: | "MedlineWanderley, Ledia Feitosa Batista, Karla Lilian Rodrigues Carvalho, Jorgiane Furtado de Lima, Aldilene da Silva Landulfo, Gabriel Alves Soares, Alexandra Martins Dos Santos Costa, Livio Martins Junior eng Brazil 2017/07/27 Rev Bras Parasitol Vet. 2017 Apr-Jun; 26(2):171-176. doi: 10.1590/S1984-29612017026" |
