| Title: | The absence of the Isw2p-Itc1p chromatin-remodelling complex induces mating type-specific and Flo11p-independent invasive growth of Saccharomyces cerevisiae |
| Author(s): | Trachtulcova P; Frydlova I; Janatova I; Hasek J; |
| Address: | "Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic" |
| ISSN/ISBN: | 0749-503X (Print) 0749-503X (Linking) |
| Abstract: | "The Isw2p-Itc1p chromatin remodelling complex of Saccharomyces cerevisiae is a member of the ISWI class of ATPases with a nucleosome spacing activity, involved in regulation of expression of a broad spectrum of genes. Its absence causes derepression of a-specific genes and aberrant morphology in alpha-mating type cells. We report here that the deletion of the ISW2 gene in the originally non-invasive BY strain induces mating type-specific invasive growth strongly affected by nitrogen starvation. Although the Flo11 protein was postulated to be critical for haploid invasive growth, we showed that the invasive growth caused by the isw2 and itc1 deletions in alpha-mating type cells was Flo11p-independent. This type of invasive growth was proved to be a consequence of the activation of the pheromone response pathway. Our results suggest that Isw2 and Itc1 proteins do not have the same impact on the described phenomenon" |
| Keywords: | "Adenosine Triphosphatases/genetics/*physiology Base Sequence Chromatin Assembly and Disassembly DNA, Fungal/genetics Gene Deletion Genes, Fungal Genes, Mating Type, Fungal Nitrogen/metabolism Pheromones/genetics/physiology Saccharomyces cerevisiae/genetic;" |
| Notes: | "MedlineTrachtulcova, Petra Frydlova, Ivana Janatova, Ivana Hasek, Jiri eng Research Support, Non-U.S. Gov't England 2004/04/30 Yeast. 2004 Apr 15; 21(5):389-401. doi: 10.1002/yea.1105" |
