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Cell

Title:		DNA binding-induced conformational change of the yeast transcriptional activator PRTF
Author(s):		Tan S; Richmond TJ;
Address:		"Institut fur Molekularbiologie und Biophysik, Eth-Honggerberg, Zurich, Switzerland"
Journal Title:		Cell
Year:		1990
Volume:		62
Issue:		2
Page Number:		367 - 377
DOI:		10.1016/0092-8674(90)90373-m
ISSN/ISBN:		0092-8674 (Print) 0092-8674 (Linking)
Abstract:		"Our studies using proteases to probe protein structure establish that binding to the upstream activating sequences (UASs) of two different yeast a-specific genes induces a conformational change in the pheromone/receptor transcription factor (PRTF), which is not observed upon binding to the UASs of either of two alpha-specific genes. We propose that this selective structural alteration exposes an activation region of PRTF when it binds a-specific genes, switching these genes on. The transcriptional activator MAT alpha 1 may activate alpha-specific genes by binding to the PRTF-alpha-specific UAS complex and unmasking the otherwise hidden activation surface of PRTF. We also show that the N-terminal third of PRTF is sufficient for specific DNA binding, while the middle third of the protein interacts with MAT alpha 1"
Keywords:		"Affinity Labels DNA/metabolism DNA-Binding Proteins/*physiology Fungal Proteins/genetics/metabolism/*ultrastructure Gene Expression Regulation, Fungal Macromolecular Substances Mating Factor Peptide Fragments/metabolism Peptides/metabolism Protein Conform;"
Notes:		"MedlineTan, S Richmond, T J eng Research Support, U.S. Gov't, Non-P.H.S. 1990/07/27 Cell. 1990 Jul 27; 62(2):367-77. doi: 10.1016/0092-8674(90)90373-m"