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Methods Mol Biol

Title:		In Situ Assays of Chemotropism During Yeast Mating
Author(s):		Stone DE; Arkowitz RA;
Address:		"Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA. dstone@uic.edu. Institute of Biology Valrose, CNRS/INSERM/Universite Nice-Sophia Antipolis, Valrose Parc, 06108, Nice Cedex 2, France. arkowitz@unice.fr"
Journal Title:		Methods Mol Biol
Year:		2016
Volume:		1407
Issue:
Page Number:		1 - 12
DOI:		10.1007/978-1-4939-3480-5_1
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Virtually all eukaryotic cells can grow in a polarized fashion in response to external signals. Cells can respond to gradients of chemoattractants or chemorepellents by directional growth, a process referred to as chemotropism. The budding yeast Saccharomyces cerevisiae undergoes chemotropic growth during mating, in which two haploid cells of opposite mating type grow towards one another. Mating pheromone gradients are essential for efficient mating in yeast and different yeast mutants are defective in chemotropism. Two methods of assessing the ability of yeast strains to respond to pheromone gradients are presented here"
Keywords:		Mating Factor/genetics/metabolism Mutation Phenotype Pheromones/*physiology Saccharomyces cerevisiae/*isolation & purification/*physiology Bud scar Chemotropism Gradient Mating pheromone Zygote;
Notes:		"MedlineStone, David E Arkowitz, Robert A eng 2016/06/09 Methods Mol Biol. 2016; 1407:1-12. doi: 10.1007/978-1-4939-3480-5_1"