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Comp Biochem Physiol B Biochem Mol Biol

Title:		Comparative analysis of expression profiling of the trypsin and chymotrypsin genes from Lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors
Author(s):		Souza TP; Dias RO; Castelhano EC; Brandao MM; Moura DS; Silva-Filho MC;
Address:		"Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Av. Padua Dias, 11, 13400-918 Piracicaba, SP, Brazil. Centro de Biologia Molecular e Engenharia Genetica, Universidade Estadual de Campinas, Av. Candido Rondon, 400, 13083-875 Campinas, SP, Brazil. Departamento de Ciencias Biologicas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Av. Padua Dias, 11, 13400-918 Piracicaba, SP, Brazil. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Av. Padua Dias, 11, 13400-918 Piracicaba, SP, Brazil. Electronic address: mdcsilva@usp.br"
Journal Title:		Comp Biochem Physiol B Biochem Mol Biol
Year:		2016
Volume:		20160302
Issue:
Page Number:		67 - 73
DOI:		10.1016/j.cbpb.2016.02.007
ISSN/ISBN:		1879-1107 (Electronic) 1096-4959 (Linking)
Abstract:		"Peptidase inhibitors (PIs) are essential proteins involved in plant resistance to herbivorous insects, yet many insect species are able to escape the negative effects of these molecules. We compared the effects of acute and chronic ingestion of soybean peptidase inhibitors (SPIs) on Spodoptera frugiperda and Diatraea saccharalis, two Lepidoptera species with different sensitivities to SPI ingestion. We analyzed the trypsin and chymotrypsin gene expression profiles in both species. Acute exposure of S. frugiperda to the inhibitors activated seven genes (SfChy5, SfChy9, SfChy19, SfChy22, SfTry6, SfTry8, and SfTry10), whereas chronic exposure activated 16 genes (SfChy2, SfChy4, SfChy5, SfChy8, SfChy9, SfChy11, SfChy12, SfChy15, SfChy17, SfChy21, SfChy22, SfTry6, SfTry8, SfTry9, SfTry10, and SfTry12). By contrast, the challenge of D. saccharalis with SPIs did not differentially induce the expression of trypsin- or chymotrypsin-encoding genes, with the exception of DsChy7. Bayesian phylogenetic analysis of S. frugiperda trypsin protein sequences revealed two gene clades: one composed of genes responsive to the SPIs and a second composed of the unresponsive genes. D. saccharalis trypsin proteins were clustered nearest to the S. frugiperda unresponsive genes. Overall, our findings support a hypothesized mechanism of resistance of Noctuidae moths to SPIs, involving gene number expansion of trypsin and chymotrypsin families and regulation of gene expression, which could also explain the variable susceptibility between S. frugiperda and D. saccharalis to these plant inhibitors"
Keywords:		"Animals Chymotrypsin/*genetics Gene Expression Regulation, Enzymologic/*drug effects Models, Molecular Protease Inhibitors/*pharmacology Protein Conformation Soybeans/*chemistry Species Specificity Spodoptera/*drug effects/enzymology/*genetics Trypsin/che;"
Notes:		"MedlineSouza, Thais P Dias, Renata O Castelhano, Elaine C Brandao, Marcelo M Moura, Daniel S Silva-Filho, Marcio C eng Comparative Study England 2016/03/06 Comp Biochem Physiol B Biochem Mol Biol. 2016 Jun-Jul; 196-197:67-73. doi: 10.1016/j.cbpb.2016.02.007. Epub 2016 Mar 2"