| Title: | CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops |
| Author(s): | Sirohi U; Kumar M; Sharma VR; Teotia S; Singh D; Chaudhary V; Priya; Yadav MK; |
| Address: | "National Institute of Plant Genome Research (NIPGR), New Delhi, 110067, India. sirohiujjwal@gmail.com. Department of Agricultural Biotechnology, College of Agriculture, SVPUAT, Meerut, Uttar Pradesh, 250110, India. sirohiujjwal@gmail.com. Department of Horticulture, College of Agriculture, SVPUAT, Meerut, Uttar Pradesh, 250110, India. Plant Genetic Resources and Improvement, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, 226001, India. Department of Biotechnology, Sharda University, Greater Noida, Uttar Pradesh, 201306, India. School of Biotechnology, Gautam Buddha University, Gautam Budh Nagar, Greater Noida, Uttar Pradesh, 201308, India. Department of Chemistry, Meerut College, Meerut, Uttar Pradesh, 250003, India. Department of Chemical Technology, Institute of Chemical Technology (ICT), Mumbai, Maharashtra, 400019, India. Department of Agricultural Biotechnology, College of Agriculture, SVPUAT, Meerut, Uttar Pradesh, 250110, India. mkyadav711@gmail.com" |
| DOI: | 10.1007/s12033-022-00523-y |
| ISSN/ISBN: | 1559-0305 (Electronic) 1073-6085 (Print) 1073-6085 (Linking) |
| Abstract: | "Demand of flowers is increasing with time worldwide. Floriculture has become one of the most important commercial trades in agriculture. Although traditional breeding methods like hybridization and mutation breeding have contributed significantly to the development of important flower varieties, flower production and quality of flowers can be significantly improved by employing modern breeding approaches. Novel traits of significance have interest to consumers and producers, such as fragrance, new floral color, change in floral architecture and morphology, vase life, aroma, and resistance to biotic and abiotic stresses, have been introduced by genetic manipulation. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has recently emerged as a powerful genome-editing tool for accurately changing DNA sequences at specific locations. It provides excellent means of genetically improving floricultural crops. CRISPR/Cas system has been utilized in gene editing in horticultural cops. There are few reports on the utilization of the CRISPR/Cas9 system in flowers. The current review summarizes the research work done by employing the CRISPR/Cas9 system in floricultural crops including improvement in flowering traits such as color modification, prolonging the shelf life of flowers, flower initiation, and development, changes in color of ornamental foliage by genome editing. CRISPR/Cas9 gene editing could be useful in developing novel cultivars with higher fragrance and enhanced essential oil and many other useful traits. The present review also highlights the basic mechanism and key components involved in the CRISPR/Cas9 system" |
| Keywords: | "*CRISPR-Associated Proteins/genetics CRISPR-Cas Systems/genetics Crops, Agricultural/genetics Genome, Plant/genetics Horticulture *Oils, Volatile Plant Breeding Plants, Genetically Modified/genetics CRISPR/Cas9 Flowering plants Gene expression Genetic imp;" |
| Notes: | "MedlineSirohi, Ujjwal Kumar, Mukesh Sharma, Vinukonda Rakesh Teotia, Sachin Singh, Deepali Chaudhary, Veena Priya Yadav, Manoj Kumar eng Review Switzerland 2022/06/26 Mol Biotechnol. 2022 Dec; 64(12):1303-1318. doi: 10.1007/s12033-022-00523-y. Epub 2022 Jun 25" |
