| Title: | Development of a static headspace gas chromatographic/mass spectrometric method to analyze the level of volatile contaminants biodegradation |
| Author(s): | Sakata SK; Taniguchi S; Rodrigues DF; Urano ME; Wandermuren MN; Pellizari VH; Comasseto JV; |
| Address: | "Instituto de Quimica, Universidade de Sao Paulo, Av Prof. Lineu Prestes 748, 05508-900 Sao Paulo, Brazil. sosakata@iq.usp.br" |
| DOI: | 10.1016/j.chroma.2004.07.021 |
| ISSN/ISBN: | 0021-9673 (Print) 0021-9673 (Linking) |
| Abstract: | "Volatile compound biodegradation analysis usually requires the time-consuming step of extraction of the analytes from the matrix using organic solvents or costly radioactive-compounds. Thus, it is desirable to have a simple and fast technique to generate a good evaluation of bacterial biodegradation. The goal of this research was to develop a methodology on the basis of static headspace-gas chromatography/mass spectrometry (HS-GC/MS) to evaluate the level of volatile contaminant biodegradation. The effects of the following parameters were studied: temperature and time of equilibration. The biodegradation experiments were carried out with bacteria inoculation in mineral media in presence of volatile hydrocarbon compounds (toluene, p-xylene, nonane and naphthalene). Autoclaved inoculates were used as control and reference sample. The optimal headspace conditions were observed when the vials were heated at 80 degrees C for 20 min, the syringe at 81 degrees C and an injection volume of 0.4 mL was used. This methodology has the advantage of being relative free from matrix effects" |
| Keywords: | "Biodegradation, Environmental Gas Chromatography-Mass Spectrometry/*methods Volatilization;" |
| Notes: | "MedlineSakata, Solange K Taniguchi, Satie Rodrigues, Debora F Urano, Miriam E Wandermuren, Marcio N Pellizari, Vivian H Comasseto, Joao V eng Research Support, Non-U.S. Gov't Netherlands 2004/09/30 J Chromatogr A. 2004 Sep 3; 1048(1):67-71. doi: 10.1016/j.chroma.2004.07.021" |
