| Title: | Enhanced volatile phenols in wine fermented with Saccharomyces cerevisiae and spoiled with Pichia guilliermondii and Dekkera bruxellensis |
| Author(s): | Saez JS; Lopes CA; Kirs VC; Sangorrin MP; |
| Address: | "Laboratorio de Microbiologia y Biotecnologia, Instituto Multidisciplinario de Investigacion y Desarrollo de la Patagonia Norte (IDEPA, CONICET-Universidad Nacional del Comahue), Buenos Aires, Neuquen, Argentina" |
| DOI: | 10.1111/j.1472-765X.2010.02878.x |
| ISSN/ISBN: | 1472-765X (Electronic) 0266-8254 (Linking) |
| Abstract: | "AIMS: To investigate whether the presence of Pichia guilliermondii impacts on the production of volatile phenols from mixed wine fermentations with Dekkera bruxellensis and Saccharomyces cerevisiae. METHODS AND RESULTS: Four inoculation strategies were performed in small-scale fermentations involving P. guilliermondii, D. bruxellensis and S. cerevisiae using Syrah grape juice supplemented with 100 mg l(-1) of p-coumaric acid. High pressure liquid chromatography was used for the quantification or volatile phenols. Significant high levels of 4-ethylphenol and 4-ethylguaicol (720 and 545 microg l(-1), respectively), as well as the highest levels of 4-vinylphenol (>4500 microg l(-1)), were observed when P. guilliermondii species was inoculated from the beginning of the fermentation. CONCLUSIONS: The metabolic interaction occurring between the high vinylphenol producer species P. guilliermondii and D. bruxellensis exhibiting a high vinylphenol reductase activity resulted in an increased production of volatile phenols in wine. SIGNIFICANCE AND IMPACT OF THE STUDY: Pichia guilliermondii must be considered a very important spoilage yeast in the wine industry capable of producing large amounts of volatile phenols" |
| Keywords: | "Chromatography, High Pressure Liquid Dekkera/growth & development/*metabolism Phenols/*analysis Pichia/growth & development/*metabolism Saccharomyces cerevisiae/growth & development/*metabolism Volatile Organic Compounds/*analysis Wine/*microbiology;" |
| Notes: | "MedlineSaez, J S Lopes, C A Kirs, V C Sangorrin, M P eng Research Support, Non-U.S. Gov't England 2010/06/23 Lett Appl Microbiol. 2010 Aug; 51(2):170-6. doi: 10.1111/j.1472-765X.2010.02878.x. Epub 2010 May 28" |
