« Previous AbstractLipid peroxidation and neuroinflammation: A possible link between maternal fructose intake and delay of acquisition of neonatal reflexes in Wistar female rats    Next Abstract"Milk saturated fatty acids, odd- and branched-chain fatty acids, and isomers of C18:1, C18:2, and C18:3n-3 according to their duodenal flows in dairy cows: A meta-analysis approach" »

J Chromatogr A

Title:		Application of solid-phase microextraction and gas chromatography-mass spectrometry to the determination of volatile organic compounds in end-exhaled breath samples
Author(s):		Prado C; Marin P; Periago JF;
Address:		"Instituto de Seguridad y Salud Laboral, Apartado 35, E-30120 El Palmar, Region de Murcia, Spain. celiaa.prado@carm.es"
Journal Title:		J Chromatogr A
Year:		2003
Volume:		1011
Issue:		1-Feb
Page Number:		125 - 134
DOI:		10.1016/s0021-9673(03)01103-8
ISSN/ISBN:		0021-9673 (Print) 0021-9673 (Linking)
Abstract:		"Analysis of exhaled air is of particular interest as an indicator of health as well as a tool for the diagnosis of diseases. It is also a very attractive procedure for the biological control of the exposition to hazardous solvents. This kind of analysis presents numerous advantages over other methods, the most important being that it is not an invasive procedure and, therefore, it is well accepted and can be applied to a wide range of compounds. Furthermore, the analysis is simplified since the matrix is less complex that in the case of blood or urine. In spite of these obvious advantages and the good results obtained, analysis of exhaled air is not in daily use, probably due to the fact that there are no normalized systems of sampling, thus making the interpretation of the results difficult. In this paper, a method for the determination of tetrachloroethylene in exhaled air using solid-phase microextraction is presented. This method, which can be applied to other volatile organic compounds, was developed with special emphasis of end-exhaled breath sampling. The sample is collected in a glass tube whose ends are closed once the exhalation is finished. The tube has an orifice sealed with a septum through which the fiber is inserted. Then, the fiber is desorbed in the injector of a gas chromatograph and the analysis is accomplished using mass spectrometry for the identification and quantification of the components. The proposed system avoids the need of complex sampling equipment and allows analysis of the alveolar fraction. Additionally, the system is economical and easy to handle, thus facilitating the development of normalized methods and its routine use in field studies"
Keywords:		*Breath Tests Gas Chromatography-Mass Spectrometry/*methods Humans Volatilization;
Notes:		"MedlinePrado, C Marin, P Periago, J F eng Netherlands 2003/10/02 J Chromatogr A. 2003 Sep 5; 1011(1-2):125-34. doi: 10.1016/s0021-9673(03)01103-8"