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Food Microbiol

Title:		Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine
Author(s):		Berbegal C; Polo L; Garcia-Esparza MJ; Lizama V; Ferrer S; Pardo I;
Address:		"ENOLAB, Estructura de Recerca Interdisciplinar (ERI) BioTecMed and Departament de Microbiologia i Ecologia, Universitat de Valencia, c/ Dr. Moliner 50, 46100, Burjassot, Valencia, Spain. Electronic address: Carmen.Berbegal@uv.es. ENOLAB, Estructura de Recerca Interdisciplinar (ERI) BioTecMed and Departament de Microbiologia i Ecologia, Universitat de Valencia, c/ Dr. Moliner 50, 46100, Burjassot, Valencia, Spain. Electronic address: Lucia.Polo@uv.es. Instituto de Ingenieria de Alimentos para el Desarrollo, Universitat Politecnica de Valencia, Camino de Vera s.n., 46022, Valencia, Spain. Electronic address: mesparza@tal.upv.es. Instituto de Ingenieria de Alimentos para el Desarrollo, Universitat Politecnica de Valencia, Camino de Vera s.n., 46022, Valencia, Spain. Electronic address: vlizama@tal.upv.es. ENOLAB, Estructura de Recerca Interdisciplinar (ERI) BioTecMed and Departament de Microbiologia i Ecologia, Universitat de Valencia, c/ Dr. Moliner 50, 46100, Burjassot, Valencia, Spain. Electronic address: Sergi.Ferrer@uv.es. ENOLAB, Estructura de Recerca Interdisciplinar (ERI) BioTecMed and Departament de Microbiologia i Ecologia, Universitat de Valencia, c/ Dr. Moliner 50, 46100, Burjassot, Valencia, Spain. Electronic address: Isabel.Pardo@uv.es"
Journal Title:		Food Microbiol
Year:		2019
Volume:		20180925
Issue:
Page Number:		25 - 37
DOI:		10.1016/j.fm.2018.09.016
ISSN/ISBN:		1095-9998 (Electronic) 0740-0020 (Linking)
Abstract:		"Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains. This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells. Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy. Total sugar consumption occurred in under 60 days in all bottles, regardless of the strain used and the way they were inoculated in wine. Deposition of lees was 3-fold faster in the bottles containing immobilised cells than in those with free cells; no addition of adjuvants was necessary. The analysis of the volatile compounds of the finished sparkling wines showed significant differences in the formation of esters, acids, alcohols, aldehydes and lactones according to the yeast and the immobilisation support used. Oak chips were the more appropriate support for yeast immobilisation. No significant differences in the sensorial analysis of the sparkling wines produced by the different strategies were found"
Keywords:		"Cells, Immobilized/*metabolism/ultrastructure Cellulose/*metabolism Esters/analysis Ethanol/analysis Fermentation/*physiology Microscopy, Electron Quercus/anatomy & histology/*microbiology Saccharomyces cerevisiae/*metabolism/ultrastructure Taste Vitis/mi;"
Notes:		"MedlineBerbegal, Carmen Polo, Lucia Garcia-Esparza, Ma Jose Lizama, Victoria Ferrer, Sergi Pardo, Isabel eng England 2018/12/01 Food Microbiol. 2019 Apr; 78:25-37. doi: 10.1016/j.fm.2018.09.016. Epub 2018 Sep 25"