| Title: | A Forward Genetic Screen for Molecules Involved in Pheromone-Induced Dauer Formation in Caenorhabditis elegans |
| Author(s): | Neal SJ; Park J; DiTirro D; Yoon J; Shibuya M; Choi W; Schroeder FC; Butcher RA; Kim K; Sengupta P; |
| Address: | "Department of Biology, Brandeis University, Waltham, Massachusetts 02454 National Center for Behavioral Genomics, Brandeis University, Waltham, Massachusetts 02454. Department of Brain and Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea. Boyce Thompson Institute, Cornell University, Ithaca, New York 14853 Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853. Department of Chemistry, University of Florida, Gainesville, Florida 32611. Department of Brain and Cognitive Sciences, DGIST, Daegu 711-873, Republic of Korea sengupta@brandeis.edu khkim@dgist.ac.kr. Department of Biology, Brandeis University, Waltham, Massachusetts 02454 National Center for Behavioral Genomics, Brandeis University, Waltham, Massachusetts 02454 sengupta@brandeis.edu khkim@dgist.ac.kr" |
| ISSN/ISBN: | 2160-1836 (Electronic) 2160-1836 (Linking) |
| Abstract: | "Animals must constantly assess their surroundings and integrate sensory cues to make appropriate behavioral and developmental decisions. Pheromones produced by conspecific individuals provide critical information regarding environmental conditions. Ascaroside pheromone concentration and composition are instructive in the decision of Caenorhabditis elegans to either develop into a reproductive adult or enter into the stress-resistant alternate dauer developmental stage. Pheromones are sensed by a small set of sensory neurons, and integrated with additional environmental cues, to regulate neuroendocrine signaling and dauer formation. To identify molecules required for pheromone-induced dauer formation, we performed an unbiased forward genetic screen and identified phd (pheromone response-defective dauer) mutants. Here, we describe new roles in dauer formation for previously identified neuronal molecules such as the WD40 domain protein QUI-1 and MACO-1 Macoilin, report new roles for nociceptive neurons in modulating pheromone-induced dauer formation, and identify tau tubulin kinases as new genes involved in dauer formation. Thus, phd mutants define loci required for the detection, transmission, or integration of pheromone signals in the regulation of dauer formation" |
| Keywords: | "Animals Caenorhabditis elegans/*drug effects/*genetics/growth & development Caenorhabditis elegans Proteins/genetics/metabolism Chromosome Mapping *Genetic Association Studies Genetic Complementation Test Genetic Linkage *Genetic Testing Genome, Helminth;" |
| Notes: | "MedlineNeal, Scott J Park, JiSoo DiTirro, Danielle Yoon, Jason Shibuya, Mayumi Choi, Woochan Schroeder, Frank C Butcher, Rebecca A Kim, Kyuhyung Sengupta, Piali eng R01 GM056223/GM/NIGMS NIH HHS/ R01 GM088290/GM/NIGMS NIH HHS/ R37 GM056223/GM/NIGMS NIH HHS/ T32 GM007122/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2016/03/16 G3 (Bethesda). 2016 May 3; 6(5):1475-87. doi: 10.1534/g3.115.026450" |
