| Title: | The cell aggregating propensity of probiotic actinobacterial isolates: isolation and characterization of the aggregation inducing peptide pheromone |
| Author(s): | Muthu Selvam R; Vinothini G; Palliyarai Thaiyammal S; Latha S; Chinnathambi A; Dhanasekaran D; Padmanabhan P; Ali Alharbi S; Archunan G; |
| Address: | "a Centre for Pheromone Technology, Department of Animal Science , Bharathidasan University , Tiruchirappalli , India. b Bioprocess Technology Laboratory, Department of Microbiology , Bharathidasan University , Tiruchirappalli , India. c Department of Botany and Microbiology , College of Science, King Saud University , Riyadh , Saudi Arabia. d Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore" |
| DOI: | 10.1080/08927014.2015.1122759 |
| ISSN/ISBN: | 1029-2454 (Electronic) 0892-7014 (Linking) |
| Abstract: | "The auto-aggregating ability of a probiotic is a prerequisite for colonization and protection of the gastrointestinal tract, whereas co-aggregation provides a close interaction with pathogenic bacteria. Peptide pheromone mediated signaling has been studied in several systems. However, it has not yet been explored in prokaryotes, especially actinobacteria. Hence, in the present study, the diffusible aggregation promoting factor was purified from the culture supernatant of a potent actinobacterial probiont and characterized using 20 different actinobacterial cultures isolated from the gut region of chicken and goat. The results showed that the pheromone-like compound induces the aggregation propensity of treated isolates. The factor was found to be a heat stable, acidic pH resistant, low molecular weight peptide which enhances the biofilm forming ability of other actinobacterial isolates. The aggregation promoting factor represents a bacterial sex factor (pheromone) and its characterization confirms its usage in the probiotic formulation" |
| Keywords: | *Actinobacteria/isolation & purification/physiology Animals Biofilms/*growth & development Chickens Gastrointestinal Tract/*microbiology Goats Microbial Interactions/*physiology Peptides/chemistry/metabolism *Pheromones/chemistry/metabolism Probiotics/*me; |
| Notes: | "MedlineMuthu Selvam, Ramu Vinothini, Gopal Palliyarai Thaiyammal, Sethuramalingam Latha, Selvanathan Chinnathambi, Arunachalam Dhanasekaran, Dharumadurai Padmanabhan, Parasuraman Ali Alharbi, Sulaiman Archunan, Govindaraju eng Research Support, Non-U.S. Gov't England 2016/01/13 Biofouling. 2016; 32(1):71-9. doi: 10.1080/08927014.2015.1122759" |
