| Title: | "Effects of Serotonin on Cell Viability, Permeability of Bovine Mammary Gland Epithelial Cells and Their Transcriptome Analysis" |
| Author(s): | Lu J; Huang G; Chang X; Wei B; Sun Y; Yang Z; Zhao Y; Zhao Z; Dong G; Chen J; |
| Address: | "College of Animal Science and Technology, Southwest University, Chongqing 400715, China. Chongqing Key Laboratory of Herbivore Science, Chongqing 400715, China" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Serotonin (5-HT) has been reported to play an important role in mammary gland involution that is defined as the process through which the gland returns to a nonlactating state. However, the overall picture of the regulatory mechanisms of 5-HT and the effects of serotonylation on mammary gland involution still need to be further investigated. The current study aimed to investigate the effects of 5-HT on global gene expression profiles of bovine mammary epithelial cells (MAC-T) and to preliminarily examine whether the serotonylation involved in the mammary gland involution by using Monodansylcadaverine (MDC), a competitive inhibitor of transglutaminase 2. Results showed that a high concentration of 5-HT decreased viability and transepithelial electrical resistance (TEER) in MAC-T cells. Transcriptome analysis indicated that 2477 genes were differentially expressed in MAC-T cells treated with 200 mug/mL of 5-HT compared with the control group, and the Notch, p53, and PI3K-Akt signaling pathways were enriched. MDC influenced 5-HT-induced MAC-T cell death, fatty acid synthesis, and the formation and disruption of tight junctions. Overall, a high concentration of 5-HT is able to accelerate mammary gland involution, which may be regulated through the Notch, p53, and PI3K-Akt signaling pathways. Serotonylation is involved in bovine mammary gland involution" |
| Keywords: | "Female Animals Cattle *Serotonin/pharmacology/metabolism *Lactation Cell Survival Phosphatidylinositol 3-Kinases/metabolism Proto-Oncogene Proteins c-akt/metabolism Tumor Suppressor Protein p53/genetics/metabolism Mammary Glands, Animal/metabolism Gene Ex;" |
| Notes: | "MedlineLu, Jie Huang, Guohao Chang, Xuan Wei, Bingni Sun, Yawang Yang, Zhengguo Zhao, Yongju Zhao, Zhongquan Dong, Guozhong Chen, Juncai eng KJQN202200203/Science and Technology Research Program of Chongqing Municipal Education Commission/ CSTB2022NSCQ-MSX1319/Natural Science Foundation of Chongqing/ SWU-KW22001/Fundamental Research Funds for the Central Universities/ S202210635056/Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates/ Switzerland 2023/07/29 Int J Mol Sci. 2023 Jul 13; 24(14):11388. doi: 10.3390/ijms241411388" |
