Title: | Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in Lilium 'Siberia' |
Author(s): | Liu Y; Wang Q; Abbas F; Zhou Y; He J; Fan Y; Yu R; |
Address: | "The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China" |
ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
Abstract: | "Light is an important environmental signal that governs plant growth, development, and metabolism. Constitutive photomorphogenic 1 (COP1) is a light signaling component that plays a vital role in plant light responses. We isolated the COP1 gene (LoCOP1) from the petals of Lilium 'Siberia' and investigated its function. The LoCOP1 protein was found to be the most similar to Apostasia shenzhenica COP1. LoCOP1 was found to be an important factor located in the nucleus and played a negative regulatory role in floral scent production and emission using the virus-induced gene silencing (VIGS) approach. The yeast two-hybrid, beta-galactosidase, and bimolecular fluorescence complementation (BiFC) assays revealed that LoCOP1 interacts with LoMYB1 and LoMYB3. Furthermore, light modified both the subcellular distribution of LoCOP1 and its interactions with LoMYB1 and MYB3 in onion cells. The findings highlighted an important regulatory mechanism in the light signaling system that governs scent emission in Lilium 'Siberia' by the ubiquitination and degradation of transcription factors via the proteasome pathway" |
Keywords: | Lilium 'Siberia' LoCOP1 LoMYBs floral scent light; |
Notes: | "PubMed-not-MEDLINELiu, Yang Wang, Qin Abbas, Farhat Zhou, Yiwei He, Jingjuan Fan, Yanping Yu, Rangcai eng 31870690,31770738/National Natural Science Foundation of China/ Switzerland 2023/09/01 Plants (Basel). 2023 May 16; 12(10):2004. doi: 10.3390/plants12102004" |