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Sci Rep

Title:		A genome-wide screen of bacterial mutants that enhance dauer formation in C. elegans
Author(s):		Khanna A; Kumar J; Vargas MA; Barrett L; Katewa S; Li P; McCloskey T; Sharma A; Naude N; Nelson C; Brem R; Killilea DW; Mooney SD; Gill M; Kapahi P;
Address:		"Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, USA. Nutrition &Metabolism Center, Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA, USA. Department of Biomedical Informatics and Medical Education, University of Washington, Seattle, Washington 98195, USA. Department of Metabolism &Aging, The Scripps Research Institute- Scripps Florida, Jupiter, Florida, 33458, USA"
Journal Title:		Sci Rep
Year:		2016
Volume:		20161213
Issue:
Page Number:		38764 -
DOI:		10.1038/srep38764
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Molecular pathways involved in dauer formation, an alternate larval stage that allows Caenorhabditis elegans to survive adverse environmental conditions during development, also modulate longevity and metabolism. The decision to proceed with reproductive development or undergo diapause depends on food abundance, population density, and temperature. In recent years, the chemical identities of pheromone signals that modulate dauer entry have been characterized. However, signals derived from bacteria, the major source of nutrients for C. elegans, remain poorly characterized. To systematically identify bacterial components that influence dauer formation and aging in C. elegans, we utilized the individual gene deletion mutants in E. coli (K12). We identified 56 diverse E. coli deletion mutants that enhance dauer formation in an insulin-like receptor mutant (daf-2) background. We describe the mechanism of action of a bacterial mutant cyaA, that is defective in the production of cyclic AMP, which extends lifespan and enhances dauer formation through the modulation of TGF-beta (daf-7) signaling in C. elegans. Our results demonstrate the importance of bacterial components in influencing developmental decisions and lifespan in C. elegans. Furthermore, we demonstrate that C. elegans is a useful model to study bacterial-host interactions"
Keywords:		*Aging Animals Caenorhabditis elegans/*microbiology Escherichia coli K12/genetics/metabolism *Gene Deletion Genome-Wide Association Study;
Notes:		"MedlineKhanna, Amit Kumar, Jitendra Vargas, Misha A Barrett, LaKisha Katewa, Subhash Li, Patrick McCloskey, Tom Sharma, Amit Naude, Nicole Nelson, Christopher Brem, Rachel Killilea, David W Mooney, Sean D Gill, Matthew Kapahi, Pankaj eng R01 AG038688/AG/NIA NIH HHS/ RL1 AG032113/AG/NIA NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2016/12/14 Sci Rep. 2016 Dec 13; 6:38764. doi: 10.1038/srep38764"