| Title: | mu-Raman Determination of Essential Oils' Constituents from Distillates and Leaf Glands of Origanum Plants |
| Author(s): | Kampasakali E; Nakas A; Mertzanidis D; Kokkini S; Assimopoulou AN; Christofilos D; |
| Address: | "School of Chemical Engineering & Physics Laboratory, Faculty of Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Natural Products Research Centre of Excellence (NatPro-AUTh), Center for Interdisciplinary Research and Innovation-Aristotle University of Thessaloniki, 57001 Thessaloniki, Greece. Laboratory of Systematic Botany and Phytogeography, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece" |
| DOI: | 10.3390/molecules28031221 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "A novel, inexpensive and simple experimental setup for collecting mu-Raman spectra of volatile liquids in very small quantities was developed. It takes advantage of capillary forces to detain minute volatile liquid volumes. Spectra of volatile and even scattering or absorbing media can be measured more effectively. The method is used to facilitate the collection of intensity-consistent Raman spectra from a series of reference compounds present in Origanum essential oils, in order to quantify their constituents by multiple linear regression. Wild grown Origanum plants, collected from five different regions in Greece and taxonomically identified as O. onites, O. vulgare subsp. hirtum and O. vulgare subsp. vulgare, were appropriately distilled to acquire their essential oils. Comparison of the Raman results with those from headspace gas chromatography-mass spectrometry (HS GC-MS) confirmed the successful relative quantification of the most abundant essential oil constituents, highlighting the similarities and differences of the three Origanum taxa examined. Finally, it is demonstrated that directly measuring the leaf peltate glandular hairs yields exploitable results to identify the main components of the essential oil they contain, underlining the potential of in situ (field or industry) measurements utilizing microscope-equipped portable Raman spectrometers" |
| Keywords: | "*Origanum/chemistry *Oils, Volatile/chemistry Gas Chromatography-Mass Spectrometry Plant Leaves/chemistry Greece Origanum Raman spectroscopy essential oils headspace GC-MS in situ analysis quantification volatile liquids;" |
| Notes: | "MedlineKampasakali, Elli Nakas, Alexandros Mertzanidis, Dimitrios Kokkini, Stella Assimopoulou, Andreana N Christofilos, Dimitrios eng T1EDK-04174/European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation/ Switzerland 2023/02/12 Molecules. 2023 Jan 26; 28(3):1221. doi: 10.3390/molecules28031221" |
