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Mycopathologia

Title:		A model to evaluate the cytotoxicity of the fungal volatile organic compound 1-octen-3-ol in human embryonic stem cells
Author(s):		Inamdar AA; Moore JC; Cohen RI; Bennett JW;
Address:		"Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. inamdar@rci.rutgers.edu"
Journal Title:		Mycopathologia
Year:		2012
Volume:		20110820
Issue:		1
Page Number:		13 - 20
DOI:		10.1007/s11046-011-9457-z
ISSN/ISBN:		1573-0832 (Electronic) 0301-486X (Linking)
Abstract:		"Microbial growth in damp indoor environments has been correlated with risks to human health. This study was aimed to determine the cytotoxicity of 1-octen-3-ol ('mushroom alcohol'), a major fungal volatile organic compound (VOC) associated with mushroom and mold odors. Using an airborne exposure technique, human embryonic stem cells were exposed for 1 h to different concentrations (0-1,000 ppm) of racemic 1-octen-3-ol and its enantiomers, (R)-(-)-1-octen-3-ol and (S)-(+)-1-octen-3-ol. Cytotoxicity was assayed using both the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay and the fluorescently tagged Calcein AM-mediated 'live and dead' assay. Racemic 1-octen-3-ol and (S)-(+)-1-octen-3-ol exhibited greater cytotoxicity to the undifferentiated human cell line H1 than did (R)-(-)-1-octen-3-ol. The inhibition concentration 50 (IC(50)) values assessed by the MTS assay for racemic 1-octen-3-ol, (S)-(+)-1-octen-3-ol and (R)-(-)-1-octen-3-ol were, respectively, 109, 98, and 258 ppm. These IC(50) values were 40-80-fold lower than that of vapor phase toluene, an industrial chemical used as a positive control in this study. Our report pioneers the modeling of human embryonic stem cells as an in vitro approach to screen the potential toxicity of fungal VOCs. Human embryonic stem cells exposed to 1-octen-3-ol, and its enantiomers in the vapor phase showed more cytotoxicity than those exposed to toluene"
Keywords:		"Cell Survival/drug effects Cells, Cultured Embryonic Stem Cells/*drug effects Humans Inhibitory Concentration 50 Octanols/*toxicity Volatile Organic Compounds/*toxicity;"
Notes:		"MedlineInamdar, Arati A Moore, Jennifer C Cohen, Rick I Bennett, Joan Wennstrom eng Research Support, Non-U.S. Gov't Netherlands 2011/08/23 Mycopathologia. 2012 Jan; 173(1):13-20. doi: 10.1007/s11046-011-9457-z. Epub 2011 Aug 20"