| Title: | Enhanced biodegradation of n-hexane by Pseudomonas sp. strain NEE2 |
| Author(s): | He S; Ni Y; Lu L; Chai Q; Liu H; Yang C; |
| Address: | "Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China. LL0106@zjgsu.edu.cn. Datang Environment Industry Group Co., Ltd, Beijing, 100097, China. Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong, 525000, China. College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan, 410082, China" |
| DOI: | 10.1038/s41598-019-52661-0 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Pseudomonas sp. strain NEE2 isolated from oil-polluted soils could biodegrade n-hexane effectively. In this study, the secretory product of n-hexane biodegradation by NEE2 was extracted, characterized, and investigated on the secretory product's enhanced effect on n-hexane removal. The effects of various biodegradation conditions on n-hexane removal by NEE2, including nitrogen source, pH value, and temperature were also investigated. Results showed that the secretory product lowered surface tension of water from 72 to 40 mN/m, with a critical micelle concentration of 340 mg/L, demonstrating that there existed biosurfactants in the secretory product. The secretory product at 50 mg/L enhanced n-hexane removal by 144.4% within 48 h than the control group. The optimum conditions for n-hexane removal by NEE2 were at temperature of 25-30 degrees C, pH value of 7-8, and (NH(4))(2)SO(4) as nitrogen source. Besides n-hexane, NEE2 could also utilize a variety of carbon sources. These results proved that NEE2 can consume hydrophobic volatile organic compounds (VOCs) to produce biosurfactants which can further enhance hydrophobic VOCs degradation" |
| Keywords: | "*Biodegradation, Environmental Hexanes/*metabolism Hydrogen-Ion Concentration Nitrogen/metabolism Pseudomonas/*metabolism Temperature;" |
| Notes: | "MedlineHe, Shanying Ni, Yaoqi Lu, Li Chai, Qiwei Liu, Haiyang Yang, Chunping eng Research Support, Non-U.S. Gov't England 2019/11/14 Sci Rep. 2019 Nov 12; 9(1):16615. doi: 10.1038/s41598-019-52661-0" |
