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J Oleo Sci

Title:		Isolation and Functional Characterization of New Terpene Synthase Genes from Traditional Edible Plants
Author(s):		Hattan JI; Shindo K; Sasaki T; Misawa N;
Address:		"Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University. Department of Food and Nutrition, Japan Women's University. Industrial Research Institute of Ishikawa"
Journal Title:		J Oleo Sci
Year:		2018
Volume:		67
Issue:		10
Page Number:		1235 - 1246
DOI:		10.5650/jos.ess18163
ISSN/ISBN:		1347-3352 (Electronic) 1345-8957 (Linking)
Abstract:		"Terpene synthase (TPS) genes were isolated and functionally characterized from three traditional edible plants, Acanthopanax sciadophylloides ('Koshiabura') and Acanthopanax sieboldianus ('Himeukogi'), belonging to the family Araliaceae, and Curcuma zedoaria (zedoary, 'Gajutsu'), belonging to the family Zingiberaceae. These plants emit characteristic fragrances and are used for traditional foods and folk medicines. From their fragrant tissues, i.e., sprouts of Araliaceae plants and developing rhizomes of zedoary, total RNAs were extracted and reverse transcribed. The resultant cDNAs were used for degenerate PCR followed by rapid amplification of cDNA ends. From the contig sequences obtained, full-length Tps genes were amplified by PCR with newly synthesized primer sets. The isolated full-length genes were introduced into engineered Escherichia coli cells, which can utilize acetoacetate to synthesize farnesyl diphosphate, the substrate for TPSs, through the mevalonate pathway. TPS products synthesized in the transformed E. coli cells were analysed by gas chromatography-mass spectrometry, nuclear magnetic resonance, and optical rotation. Consequently, the isolated Tps genes were found to encode beta-caryophyllene synthase, germacrene D synthase, linalool/(3S)-(+)-nerolidol synthase, beta-eudesmol synthase, and germacrene B synthase. These results lead us to expect that some of the effective ingredients in folk medicines are volatile terpenes and that intake of traditional foods including these edible plants would have some positive effects on our health"
Keywords:		"Acetoacetates/metabolism Alkyl and Aryl Transferases/*genetics/*isolation & purification Curcuma/chemistry/*enzymology/*genetics DNA, Complementary Eleutherococcus/chemistry/*enzymology/*genetics Escherichia coli/metabolism Mevalonic Acid/metabolism Plant;"
Notes:		"MedlineHattan, Jun-Ichiro Shindo, Kazutoshi Sasaki, Tetsuya Misawa, Norihiko eng Japan 2018/10/12 J Oleo Sci. 2018; 67(10):1235-1246. doi: 10.5650/jos.ess18163"