| Title: | Detection of Volatile Organic Compounds as Potential Novel Biomarkers for Chorioamnionitis - Proof of Experimental Models |
| Author(s): | Goedicke-Fritz S; Werner T; Niemarkt HJ; Wolfs T; Baumbach JI; Kemp MW; Jobe AH; Rogosch T; Bous M; Kaiser E; Stutz R; Meyer S; Maier RF; Koczulla AR; Spiller OB; Kramer BW; Zemlin M; |
| Address: | "Children's Hospital, Philipps University, Marburg, Germany. Department of General Paediatrics and Neonatology, Saarland University Medical School, Homburg, Germany. Department of Neonatology, Maxima Medical Centre, Veldhoven, Netherlands. Department of Pediatrics, School of Oncology and Developmental Biology, Maastricht University, Maastricht, Netherlands. Center of Competence Breath Analysis, Branch Dortmund, B. Braun Melsungen AG, Melsungen, Germany. Division of Obstetrics and Gynecology, School of Women's and Infants' Health, University of Western Australia, Crawley, WA, Australia. Department of Pulmonology, German Center of Lung Research DZL, Institute for Internal Medicine, Philipps-University of Marburg, Marburg, Germany. Department of Microbiology, School of Medicine, Cardiff University, Cardiff, United Kingdom" |
| ISSN/ISBN: | 2296-2360 (Print) 2296-2360 (Electronic) 2296-2360 (Linking) |
| Abstract: | "Background: Histologic chorioamnionitis is only diagnosed postnatally which prevents interventions. We hypothesized that volatile organic compounds (VOCs) in the amniotic fluid might be useful biomarkers for chorioamnionitis and that VOC profiles differ between amnionitis of different origins. Methods: Time-mated ewes received intra-amniotic injections of media or saline (controls), or live Ureaplasma parvum serovar 3 (Up) 14, 7 or 3d prior to c-section at day 124 gestational age (GA). 100 mug recombinant ovine IL-1alpha was instilled at 7, 3 or 1d prior to delivery. Headspace VOC profiles were measured from amniotic fluids at birth using ion mobility spectrometer coupled with multi-capillary columns. Results: 127 VOC peaks were identified. 27 VOCs differed between samples from controls and Up- or IL-1alpha induced amnionitis. The best discrimination between amnionitis by Up vs. IL-1alpha was reached by 2-methylpentane, with a sensitivity/specificity of 96/95% and a positive predictive value/negative predictive values of 96 and 95%. The concentration of 2-methylpentane in VOCs peaked 7d after intra-amniotic instillation of Up. Discussion: We established a novel method to study headspace VOC profiles of amniotic fluids. VOC profiles may be a useful tool to detect and to assess the duration of amnionitis induced by Up. 2-methylpentane was previously described in the exhalate of women with pre-eclampsia and might be a volatile biomarker for amnionitis. Amniotic fluids analyzed by ion mobility spectrometry coupled with multi-capillary columns may provide bedside diagnosis of amnionitis and understanding inflammatory mechanisms during pregnancy" |
| Keywords: | antenatal inflammation detection duration infection ureaplasma volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINEGoedicke-Fritz, Sybelle Werner, Thomas Niemarkt, Hendrik J Wolfs, Tim G A M Baumbach, Jorg Ingo Kemp, Matthew W Jobe, Alan H Rogosch, Tobias Bous, Michelle Kaiser, Elisabeth Stutz, Regine Meyer, Sascha Maier, Rolf Felix Koczulla, Andreas Rembert Spiller, Owen Brad Kramer, Boris W Zemlin, Michael eng Switzerland 2021/08/10 Front Pediatr. 2021 Jul 22; 9:698489. doi: 10.3389/fped.2021.698489. eCollection 2021" |
