| Title: | "1-Octanol, a self-inhibitor of spore germination in Penicillium camemberti" |
| Author(s): | Gillot G; Decourcelle N; Dauer G; Barbier G; Coton E; Delmail D; Mounier J; |
| Address: | "Universite de Brest, EA 3882 Laboratoire Universitaire de Biodiversite et Ecologie Microbienne, ESIAB, Technopole Brest-Iroise, 29280, Plouzane, France. Universite de Brest, EA 3882 Laboratoire Universitaire de Biodiversite et Ecologie Microbienne, IUT de Quimper, 2, Rue de L'Universite, 29200, Quimper, France. Universite de Rennes 1, UEB, UMR CNRS 6226 ISCR PNSCM, 2 Avenue Du Professeur Leon Bernard, 35043, Rennes, France. Universite de Brest, EA 3882 Laboratoire Universitaire de Biodiversite et Ecologie Microbienne, ESIAB, Technopole Brest-Iroise, 29280, Plouzane, France. Electronic address: jerome.mounier@univ-brest.fr" |
| ISSN/ISBN: | 1095-9998 (Electronic) 0740-0020 (Linking) |
| Abstract: | "Penicillium camemberti is a technologically relevant fungus used to manufacture mold-ripened cheeses. This fungal species produces many volatile organic compounds (VOCs) including ammonia, methyl-ketones, alcohols and esters. Although it is now well known that VOCs can act as signaling molecules, nothing is known about their involvement in P. camemberti lifecycle. In this study, spore germination was shown to be self-regulated by quorum sensing in P. camemberti. This phenomenon, also called 'crowding effect', is population-dependent (i.e. observed at high population densities). After determining the volatile nature of the compounds involved in this process, 1-octanol was identified as the main compound produced at high-spore density using GC-MS. Its inhibitory effect was confirmed in vitro and 3 mM 1-octanol totally inhibited spore germination while 100 muM only transiently inhibited spore germination. This is the first time that self-inhibition of spore germination is demonstrated in P. camemberti. The obtained results provide interesting perspectives for better control of mold-ripened cheese processes" |
| Keywords: | "1-Octanol/analysis/*metabolism Antifungal Agents/analysis/*metabolism Cheese/microbiology Gas Chromatography-Mass Spectrometry Penicillium/growth & development/*metabolism Spores, Fungal/*growth & development/metabolism Volatile Organic Compounds/analysis;" |
| Notes: | "MedlineGillot, Guillaume Decourcelle, Nicolas Dauer, Gaelle Barbier, Georges Coton, Emmanuel Delmail, David Mounier, Jerome eng Research Support, Non-U.S. Gov't England 2016/04/08 Food Microbiol. 2016 Aug; 57:1-7. doi: 10.1016/j.fm.2015.12.008. Epub 2015 Dec 30" |
