| Title: | Comparative evaluation of an ISO 3632 method and an HPLC-DAD method for safranal quantity determination in saffron |
| Author(s): | Garcia-Rodriguez MV; Lopez-Corcoles H; Alonso GL; Pappas CS; Polissiou MG; Tarantilis PA; |
| Address: | "Catedra de Quimica Agricola, E.T.S.I. Agronomos, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, Spain. Instituto Tecnico Agronomico Provincial (ITAP), S.A. Avda. Gregorio Arcos, s/n., 02006 Albacete, Spain. Laboratory of Chemistry, Department of Food Science and Human Nutrition, School of Food, Biotechnology and Development, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece. Laboratory of Chemistry, Department of Food Science and Human Nutrition, School of Food, Biotechnology and Development, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece. Electronic address: ptara@aua.gr" |
| DOI: | 10.1016/j.foodchem.2016.11.089 |
| ISSN/ISBN: | 1873-7072 (Electronic) 0308-8146 (Linking) |
| Abstract: | "The aim of this work was a comparison of the ISO 3632 (2011) method and an HPLC-DAD method for safranal quantity determination in saffron. Samples from different origins were analysed by UV-vis according to ISO 3632 (2011) and by HPLC-DAD. Both methods were compared, and there was no correlation between the safranal content obtained by UV-vis and HPLC-DAD. An over-estimation in the UV-vis experiment was observed, which was related to the cis-crocetin esters content, as well as other compounds. The results demonstrated that there was no relationship between ISO quality categories and safranal content using HPLC-DAD. Therefore, HPLC-DAD might be preferable to UV-vis for determining the safranal content and the classification of saffron for commercial purposes. In addition, HPLC-DAD was adequate for determining the three foremost parameters that define the quality of saffron (crocetin esters, picrocrocin and safranal); therefore, this approach could be included in the ISO 3632 method (2011)" |
| Keywords: | "Carotenoids/analysis *Chromatography, High Pressure Liquid Crocus/*chemistry Cyclohexenes/*analysis Esters/analysis Food Analysis/*methods Food Quality Glucosides/analysis Plant Extracts/chemistry Terpenes/*analysis *Ultraviolet Rays Vitamin A/analogs & d;" |
| Notes: | "MedlineGarcia-Rodriguez, M Valle Lopez-Corcoles, Horacio Alonso, Gonzalo L Pappas, Christos S Polissiou, Moschos G Tarantilis, Petros A eng Comparative Study England 2016/12/17 Food Chem. 2017 Apr 15; 221:838-843. doi: 10.1016/j.foodchem.2016.11.089. Epub 2016 Nov 21" |
