| Title: | Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from Allium spp |
| Author(s): | Garcia-Nicolas M; Pastor-Belda M; Campillo N; Rodriguez-Sojo MJ; Ruiz-Malagon AJ; Hidalgo-Garcia L; Abad P; de la Torre JM; Guillamon E; Banos A; Galvez J; Vinas P; Arroyo-Manzanares N; |
| Address: | "Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, E-30100 Murcia, Spain. Department of Pharmacology, Instituto de Investigacion Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, Spain. Department of Inorganic and Organic Chemistry, Campus of Lagunillas, Universidad de Jaen, E-23071 Jaen, Spain. Department of Microbiology, University of Granada, Fuente Nueva s/n, E-19071 Granada, Spain" |
| ISSN/ISBN: | 2304-8158 (Print) 2304-8158 (Electronic) 2304-8158 (Linking) |
| Abstract: | "The present work is focused on the development of an analytical platform to elucidate the metabolic pathway of PTSO from onion, an organosulfur compound well-known for its functional and technological properties and its potential application in animal and human nutrition. This analytical platform consisted of the use of gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography quadrupole with time-of-flight MS (UHPLC-Q-TOF-MS) in order to monitor volatile and non-volatile compounds derived from the PTSO. For the extraction of the compounds of interest, two different sample treatments were developed: liquid-liquid extraction (LLE) and salting-out assisted liquid-liquid extraction (SALLE) for GC-MS and UHPLC-Q-TOF-MS analysis, respectively. Once the analytical platform was optimised and validated, an in vivo study was planned to elucidate PTSO metabolisation, revealing the presence of dipropyl disulfide (DPDS) in liver samples with concentrations between 0.11 and 0.61 microg g(-1). The DPDS maximum concentration in the liver was observed at 0.5 h after the intake. DPDS was also present in all plasma samples with concentrations between 2.1 and 2.4 microg mL(-1). In regard to PTSO, it was only found in plasma at times above 5 h (0.18 microg mL(-1)). Both PTSO and DPDS were excreted via urine 24 h after ingestion" |
| Keywords: | analytical platform in vivo study metabolisation propyl propane thiosulfonate rats; |
| Notes: | "PubMed-not-MEDLINEGarcia-Nicolas, Maria Pastor-Belda, Marta Campillo, Natalia Rodriguez-Sojo, Maria Jesus Ruiz-Malagon, Antonio Jesus Hidalgo-Garcia, Laura Abad, Paloma de la Torre, Jose Manuel Guillamon, Enrique Banos, Alberto Galvez, Julio Vinas, Pilar Arroyo-Manzanares, Natalia eng PGC2018-098363-B-100/Spanish MICINN/ R-1328/2018/University of Murcia/ Switzerland 2023/02/26 Foods. 2023 Feb 15; 12(4):823. doi: 10.3390/foods12040823" |
