| Title: | "Isolation, Expression Profiling, and Regulation via Host Allelochemicals of 16 Glutathione S-Transferases in the Chinese White Pine Beetle, Dendroctonus armandi" |
| Author(s): | Gao H; Dai L; Fu D; Sun Y; Chen H; |
| Address: | "College of Forestry, Northwest A&F University, Yangling, China. College of Forestry, Nanjing Forestry University, Nanjing, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China" |
| DOI: | 10.3389/fphys.2020.546592 |
| ISSN/ISBN: | 1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking) |
| Abstract: | "The Chinese white pine beetle (Dendroctonus armandi) is undoubtedly one of the most important pests causing ecological damage in the Qinling Mountains. When bark beetles invade conifers, they must overcome host tree defenses, including primary resistance and induced resistance responses. Moreover, this induced resistance occurs following herbivory by bark beetles. Bark beetles have a corresponding defense mechanism for degrading toxic compounds, and glutathione S-transferases (GSTs) can catalyze the binding of endogenous substances that reduce glutathione (GSH) to various harmful electrophilic substrates, increasing their solubility and facilitating their excretion from cells. In this experiment, we successfully obtained sixteen full-length sequences of D. armandi, which belonged to four GST categories (delta, epsilon, sigma, and theta). The transcript levels of sixteen GSTs in D. armandi were compared at four developmental stages (larvae, pupae, teneral adults, and adults), three different tissues (antennae, gut, and reproductive organs), and under various levels of terpenoid stimuli during feeding on phloem tissue to evaluate the various relevant modes of action. This study aids in the understanding of the interaction between monoterpenes and beetles, and beetles' detoxification through GSTs" |
| Keywords: | Chinese white pine beetle glutathione S-transferases host phloem terpenoids transcript levels; |
| Notes: | "PubMed-not-MEDLINEGao, Haiming Dai, Lulu Fu, Danyang Sun, Yaya Chen, Hui eng Switzerland 2020/12/08 Front Physiol. 2020 Nov 12; 11:546592. doi: 10.3389/fphys.2020.546592. eCollection 2020" |
