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FEMS Microbiol Lett

Title:		Molecular monitoring of the transcriptional activation of the yeast Saccharomyces kluyveri mating pheromone signal transduction by using FUS1-lacZ fusion gene
Author(s):		Fujimura H;
Address:		"Laboratory for Molecular Biology, Hoechst Japan Limited, Kawagoe, Japan"
Journal Title:		FEMS Microbiol Lett
Year:		1992
Volume:		73
Issue:		1-Feb
Page Number:		23 - 26
DOI:		10.1016/0378-1097(92)90576-a
ISSN/ISBN:		0378-1097 (Print) 0378-1097 (Linking)
Abstract:		"To analyse the molecular mechanism by which a mating pheromone signal is transmitted in Saccharomyces kluyveri, the S. cerevisiae FUS1-lacZ fusion gene was introduced into S. kluyveri cells and its transcriptional activation was investigated. The expression of FUS1-lacZ fusion product was cell-type-specifically induced by pheromone treatment in S. kluyveri. The result suggests that S. kluyveri pheromone signal may be transduced by a mechanism similar to that in S. cerevisiae. This system may provide a model for analysing the molecular mechanism of the mating reaction in Saccharomyces kluyveri"
Keywords:		"Conjugation, Genetic *Gene Expression Regulation, Fungal *Genes, Fungal Recombinant Fusion Proteins/biosynthesis Saccharomyces/*genetics Sex Attractants/*genetics Signal Transduction/*genetics *Transcription, Genetic beta-Galactosidase/*genetics;"
Notes:		"MedlineFujimura, H eng England 1992/07/01 FEMS Microbiol Lett. 1992 Jul 1; 73(1-2):23-6. doi: 10.1016/0378-1097(92)90576-a"