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Molecules

Title:		A Mass Spectrometry-Based Study Shows that Volatiles Emitted by Arthrobacter agilis UMCV2 Increase the Content of Brassinosteroids in Medicago truncatula in Response to Iron Deficiency Stress
Author(s):		Flores-Cortez I; Winkler R; Ramirez-Ordorica A; Elizarraraz-Anaya MIC; Carrillo-Rayas MT; Valencia-Cantero E; Macias-Rodriguez L;
Address:		"Instituto de Investigaciones Quimico Biologicas, Universidad Michoacana de San Nicolas de Hidalgo. Edifico B3, Ciudad Universitaria, Morelia 58030, Michoacan, Mexico. Department of Biotechnology and Biochemistry, Cinvestav Unidad Irapuato, Irapuato, Km 9.6 Libramiento Norte Carr. Irapuato-Leon, Guanajuato 36824, Mexico. Instituto de Investigaciones Quimico Biologicas, Universidad Michoacana de San Nicolas de Hidalgo. Edifico B3, Ciudad Universitaria, Morelia 58030, Michoacan, Mexico. lmacias@umich.mx"
Journal Title:		Molecules
Year:		2019
Volume:		20190820
Issue:		16
Page Number:		-
DOI:		10.3390/molecules24163011
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Iron is an essential plant micronutrient. It is a component of numerous proteins and participates in cell redox reactions; iron deficiency results in a reduction in nutritional quality and crop yields. Volatiles from the rhizobacterium Arthrobacter agilis UMCV2 induce iron acquisition mechanisms in plants. However, it is not known whether microbial volatiles modulate other metabolic plant stress responses to reduce the negative effect of iron deficiency. Mass spectrometry has great potential to analyze metabolite alterations in plants exposed to biotic and abiotic factors. Direct liquid introduction-electrospray-mass spectrometry was used to study the metabolite profile in Medicago truncatula due to iron deficiency, and in response to microbial volatiles. The putatively identified compounds belonged to different classes, including pigments, terpenes, flavonoids, and brassinosteroids, which have been associated with defense responses against abiotic stress. Notably, the levels of these compounds increased in the presence of the rhizobacterium. In particular, the analysis of brassinolide by gas chromatography in tandem with mass spectrometry showed that the phytohormone increased ten times in plants grown under iron-deficient growth conditions and exposed to microbial volatiles. In this mass spectrometry-based study, we provide new evidence on the role of A. agilis UMCV2 in the modulation of certain compounds involved in stress tolerance in M. truncatula"
Keywords:		"Agricultural Inoculants Arthrobacter/*metabolism Brassinosteroids/analysis/*metabolism Cluster Analysis Iron/*metabolism Medicago truncatula/drug effects/growth & development/*metabolism Models, Biological Seedlings/drug effects/growth & development/metab;"
Notes:		"MedlineFlores-Cortez, Idolina Winkler, Robert Ramirez-Ordorica, Arturo Elizarraraz-Anaya, Ma Isabel Cristina Carrillo-Rayas, Maria Teresa Valencia-Cantero, Eduardo Macias-Rodriguez, Lourdes eng 2.24/Universidad Michoacana de San Nicolas de Hidalgo/ 2015-2/814/Consejo Nacional de Ciencia y Tecnologia/ 2016/277850/Consejo Nacional de Ciencia y Tecnologia/ 164395/Consejo Nacional de Ciencia y Tecnologia/ Switzerland 2019/08/23 Molecules. 2019 Aug 20; 24(16):3011. doi: 10.3390/molecules24163011"