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Fungal Genet Biol

Title:		Dual-specificity protein phosphatase Msg5 controls cell wall integrity and virulence in Fusarium oxysporum
Author(s):		Fernandes TR; Sanchez Salvador E; Tapia AG; Di Pietro A;
Address:		"Departamento de Genetica, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain. Departamento de Genetica, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain. Electronic address: ge2dipia@uco.es"
Journal Title:		Fungal Genet Biol
Year:		2021
Volume:		20201121
Issue:
Page Number:		103486 -
DOI:		10.1016/j.fgb.2020.103486
ISSN/ISBN:		1096-0937 (Electronic) 1087-1845 (Linking)
Abstract:		"Mitogen-activated protein kinase (MAPK) cascades are key signaling modules controlling development and virulence in fungal pathogens. Down-regulation of MAPK activity by protein phosphatases provides a critical layer of control during desensitization or adaptation to stimuli. In Saccharomyces cerevisiae, the dual-specificity phosphatase Msg5 dephosphorylates target threonine and tyrosine residues in the two MAPKs Mpk1 and Fus3, which regulate the cell wall integrity (CWI) and pheromone responses, respectively. Here we studied the role of the Msg5 ortholog in Fusarium oxysporum, a soilborne phytopathogen that infects host plants through the roots to cause vascular wilt and plant death. F. oxysporum mutants lacking Msg5 showed constitutively high levels of Mpk1 phosphorylation and increased sensitivity to the cell wall targeting compound Calcofluor White. Moreover, these mutants displayed reduced colony growth and conidiation. Importantly, msg5Delta mutants were impaired in hyphal chemotropism towards host plant roots and in virulence on tomato plants. These results reveal a key role of Msg5 in regulation of the CWI MAPK cascade of F. oxysporum as well as in infection-related signaling of this important fungal pathogen"
Keywords:		Cell Wall/*genetics Dual-Specificity Phosphatases/*genetics Fusarium/*genetics/growth & development Hyphae/growth & development Solanum lycopersicum/microbiology Phosphorylation Plant Roots/microbiology Protein Tyrosine Phosphatases/genetics Saccharomyces;
Notes:		"MedlineFernandes, Tania R Sanchez Salvador, Encarnacion Tapia, Angela G Di Pietro, Antonio eng Research Support, Non-U.S. Gov't 2020/11/25 Fungal Genet Biol. 2021 Jan; 146:103486. doi: 10.1016/j.fgb.2020.103486. Epub 2020 Nov 21"