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Int J Mol Sci

Title:		miR-450-5p and miR-202-5p Synergistically Regulate Follicle Development in Black Goat
Author(s):		Feng G; Liu J; Lu Z; Li Y; Deng M; Liu G; Sun B; Guo Y; Zou X; Liu D;
Address:		"Herbivore Laboratory, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China. National Joint Engineering Research Center, South China Agricultural University, Guangzhou 510642, China. Guangdong Key Laboratory of Agricultural Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China. Collaborative Innovation Center for Healthy Sheep Breeding and Zoonoses Prevention and Control, Shihezi University, Shihezi 832000, China"
Journal Title:		Int J Mol Sci
Year:		2022
Volume:		20221226
Issue:		1
Page Number:		-
DOI:		10.3390/ijms24010401
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Follicle maturation is a complex biological process governed by numerous factors, and researchers have observed follicle development by studying the proliferation and apoptosis of follicular granulosa cells (GCs). However, the regulatory mechanisms of GCs proliferation and death during follicle development are largely unknown. To investigate the regulatory mechanisms of lncRNAs, mRNAs, and microRNAs, RNA sequencing (RNA-seq) and small RNA-seq were performed on large (>10 mm) and small follicles (<3 mm) of Leizhou black goat during estrus. We discovered two microRNAs, miR-450-5p and miR-202-5p, which can target GCs in goats and may be involved in follicle maturation, and the effects of miR-450-5p and miR-202-5p on ovarian granulosa cell lines were investigated (KGN). Using cell counting kit-8 (CCK-8) assays, 5-Ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry, miR-202-5p overexpression could suppress the proliferation and induce apoptosis of GCs, whereas miR-450-5p overexpression induced the opposite effects. The dual-luciferase reporter assay confirmed that miR-450-5p could directly target the BMF gene (a BCL2 modifying factor), and miR-202-5p targeted the BCL2 gene. A considerable rise in phosphorylated Akt (p-AKT) protein was observed following the downregulation of BMF by miR-450-5p mimics. After BMF gene RNAi therapy, a notable elevation in p-AKT was detected. Mimics of miR-202-5p inhibited BCL2 protein expression, significantly decreasing p-AMPK protein expression. These results imply that during the follicular development in black goats, the miR-450-5p-BMF axis favored GC proliferation on a wide scale, while the miR-202-5p-BCL2 axis triggered GC apoptosis"
Keywords:		Animals Female Apoptosis/genetics Cell Proliferation/genetics Goats/genetics/metabolism *MicroRNAs/genetics/metabolism Proto-Oncogene Proteins c-akt Proto-Oncogene Proteins c-bcl-2 *Ovarian Follicle/growth & development MiR-202-5p MiR-450-5p follicle deve;
Notes:		"MedlineFeng, Guanghang Liu, Jie Lu, Zitao Li, Yaokun Deng, Ming Liu, Guangbin Sun, Baoli Guo, Yongqing Zou, Xian Liu, Dewu eng 2021KJ127/Modern Agricultural Industrial Technology System of Guangdong Province/ 2022B0202110002/Key Realm R& D Program of GuangDong Province/ 2021A1515010636/Natural Science Foundation of Guangdong Province/ Switzerland 2023/01/09 Int J Mol Sci. 2022 Dec 26; 24(1):401. doi: 10.3390/ijms24010401"