| Title: | A 3 kDa peptide is involved in the chemoattraction in vitro of the male Schistosoma mansoni to the female |
| Author(s): | Ghaleb AM; Atwood J; Morales-Montor J; Damian RT; |
| Address: | "Division of Digestive Diseases, R275, WBRB, Emory University School of Medicine, 615 Michael St., Atlanta, GA 30322, USA" |
| DOI: | 10.1016/j.micinf.2006.04.024 |
| ISSN/ISBN: | 1286-4579 (Print) 1286-4579 (Linking) |
| Abstract: | "In vitro experiments on adult Schistosoma mansoni worms have shown the existence of mutual attraction between males and females mediated by factor(s) released by both sexes. Males were attracted to female-released factor(s), which was found in <30 kDa fractions. A combination of bioassays, size fractionations, chromatographic and chemical and enzymatic treatments were used to identify female-released factor(s) that attracted males. Boiling reduced the attraction of males towards female incubate. Male-attractant factor(s) was not in the female vomitus, and TLC showed the absence of lipids and carbohydrates in the female incubate. Male-attractant factor(s) was found to be sensitive to proteases and to be hydrophilic in nature. Using RP-HPLC, a 3260 Da peptide released by S. mansoni adult females that plays a role in attracting the males was identified, purified and partially characterized. A partial amino acid sequence of that peptide was obtained using MALDI peptide-MS and MALDI-TOF/TOF" |
| Keywords: | "Amino Acid Sequence Animals Chromatography, High Pressure Liquid/methods Female Male Mice Mice, Inbred C57BL Mice, Inbred CBA Molecular Sequence Data Peptides/*chemistry/isolation & purification/physiology Schistosoma mansoni/*chemistry/physiology Sex Att;" |
| Notes: | "MedlineGhaleb, Amr M Atwood, James 3rd Morales-Montor, Jorge Damian, Raymond T eng Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't France 2006/07/20 Microbes Infect. 2006 Aug; 8(9-10):2367-75. doi: 10.1016/j.micinf.2006.04.024. Epub 2006 Jul 3" |
