« Previous Abstract"The human volatilome: volatile organic compounds (VOCs) in exhaled breath, skin emanations, urine, feces and saliva"    Next AbstractGC-MS-olfactometric characterization of the most aroma-active components in a representative aromatic extract from Iranian saffron (Crocus sativus L.) »

J Insect Sci

Title:		A Diagnostic Loop-Mediated Isothermal Amplification Method to Distinguish Helicoverpa armigera (Lepidoptera: Noctuidae) From Other Related Species in the New World
Author(s):		Amano T; Nomura M;
Address:		"Laboratory of Applied Entomology, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan"
Journal Title:		J Insect Sci
Year:		2020
Volume:		20
Issue:		3
Page Number:		-
DOI:		10.1093/jisesa/ieaa046
ISSN/ISBN:		1536-2442 (Electronic) 1536-2442 (Linking)
Abstract:		"Helicoverpa armigera (Hubner) is a notorious agricultural pest native to the Old World. Recently, its invasion into South and Central America has become a serious problem in the New World. The rapid detection of invasive pests is essential to eradicate them and prevent establishment. However, an extremely similar species, H. zea (Boddie) distributed in the New World makes identification difficult. Helicoverpa armigera and H. zea have only minor differences in male genitalia to separate them morphologically. Both species are attracted to the same pheromone lure, and it takes considerable time and effort to identify them from bulk samples obtained during trap monitoring. Although several molecular approaches based on PCR have been reported, these methods require expensive equipment and are unsuitable for onsite diagnostics. Here, we developed a rapid and convenient diagnostic method based on the loop-mediated isothermal amplification to distinguish H. armigera from related species: H. zea, H. assulta (Guenee), H. punctigera (Wallengren), and Chloridea virescens (Fabricius). The diagnostic method makes it possible to detect H. armigera within 90 min only using simple equipment. The method also worked with mixed DNA templates containing excess DNA from H. zea at the ratio of 1:999 (H. armigera:H. zea). This method can be an effective tool for onsite diagnostics during monitoring surveys for invasive H. armigera"
Keywords:		Animals Insect Control/*methods Moths/*classification/genetics Nucleic Acid Amplification Techniques/*methods Helicoverpa loop-mediated isothermal amplification molecular identification;
Notes:		"MedlineAmano, Takayuki Nomura, Masashi eng 2020/05/28 J Insect Sci. 2020 May 1; 20(3):10. doi: 10.1093/jisesa/ieaa046"