| Title: | Analysis of a new cluster of genes involved in the synthesis of the unique volatile organic compound sodorifen of Serratia plymuthica 4Rx13 |
| Author(s): | Domik D; Magnus N; Piechulla B; |
| Address: | "Institute for Biological Sciences, University of Rostock, Albert-Einstein-Strasse 3, 18059 Rostock, Germany. Institute for Biological Sciences, University of Rostock, Albert-Einstein-Strasse 3, 18059 Rostock, Germany birgit.piechulla@uni-rostock.de" |
| ISSN/ISBN: | 1574-6968 (Electronic) 0378-1097 (Linking) |
| Abstract: | "The rhizobacterium Serratia plymuthica 4Rx13 emits the novel and unique volatile sodorifen (C16H26), which has a polymethylated bicyclic structure. Transcriptome analysis revealed that gene SOD_c20750 (annotated as terpene cyclase) is involved in the biosynthesis of sodorifen. Here we show that this gene is located in a small cluster of four genes (SOD_c20750 - SOD_c20780), and the analysis of the knockout mutants demonstrated that SOD_c20760 (annotated as methyltransferase) and SOD_c20780 (annotated as isopentenyl pyrophosphate (IPP) isomerase) are needed for the biosynthesis of sodorifen, while a sodorifen-negative phenotype was not achieved with the SOD_c20770 (annotated as deoxy-xylulose-5-phosphate (DOXP) synthase) mutant. Altogether, the function of this new gene cluster was assigned to the biosynthesis of this structurally unusual volatile compound sodorifen" |
| Keywords: | "Bridged Bicyclo Compounds/*metabolism Gene Expression Profiling Gene Knockout Techniques Gene Order *Genes, Bacterial Genome, Bacterial *Multigene Family Octanes/*metabolism Serratia/*genetics/*metabolism Transcriptome Volatile Organic Compounds/*metaboli;" |
| Notes: | "MedlineDomik, Dajana Magnus, Nancy Piechulla, Birgit eng Research Support, Non-U.S. Gov't England 2016/05/28 FEMS Microbiol Lett. 2016 Jul; 363(14):fnw139. doi: 10.1093/femsle/fnw139. Epub 2016 May 25" |
