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ISME J

Title:		Microbial methanol uptake in northeast Atlantic waters
Author(s):		Dixon JL; Beale R; Nightingale PD;
Address:		"Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth, Devon, UK. jod@pml.ac.uk"
Journal Title:		ISME J
Year:		2011
Volume:		20101111
Issue:		4
Page Number:		704 - 716
DOI:		10.1038/ismej.2010.169
ISSN/ISBN:		1751-7370 (Electronic) 1751-7362 (Print) 1751-7362 (Linking)
Abstract:		"Methanol is the predominant oxygenated volatile organic compound in the troposphere, where it can significantly influence the oxidising capacity of the atmosphere. However, we do not understand which processes control oceanic concentrations, and hence, whether the oceans are a source or a sink to the atmosphere. We report the first methanol loss rates in seawater by demonstrating that (14)C-labelled methanol can be used to determine microbial uptake into particulate biomass, and oxidation to (14)CO(2). We have found that methanol is used predominantly as a microbial energy source, but also demonstrated its use as a carbon source. We report biological methanol oxidation rates between 2.1 and 8.4 nmol l(-1) day(-1) in surface seawater of the northeast Atlantic. Kinetic experiments predict a V(max) of up to 29 nmol l(-1) day(-1), with a high affinity K(m) constant of 9.3 nM in more productive coastal waters. We report surface concentrations of methanol in the western English channel of 97+/-8 nM (n=4) between May and June 2010, and for the wider temperate North Atlantic waters of 70+/-13 nM (n=6). The biological turnover time of methanol has been estimated between 7 and 33 days, although kinetic experiments suggest a 7-day turnover in more productive shelf waters. Methanol uptake rates into microbial particles significantly correlated with bacterial and phytoplankton parameters, suggesting that it could be used as a carbon source by some bacteria and possibly some mixotrophic eukaryotes. Our results provide the first methanol loss rates from seawater, which will improve the understanding of the global methanol budget"
Keywords:		Bacteria/*metabolism Biomass Methanol/*metabolism Oceans and Seas Phytoplankton/metabolism Seawater/*microbiology Water Microbiology;
Notes:		"MedlineDixon, Joanna L Beale, Rachael Nightingale, Philip D eng Research Support, Non-U.S. Gov't England 2010/11/12 ISME J. 2011 Apr; 5(4):704-16. doi: 10.1038/ismej.2010.169. Epub 2010 Nov 11"