| Title: | Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape |
| Author(s): | Di Francesco A; Zajc J; Gunde-Cimerman N; Aprea E; Gasperi F; Placi N; Caruso F; Baraldi E; |
| Address: | "CRIOF-Department of Agricultural Sciences, University of Bologna, Via Gandolfi, 19, 40057, Cadriano, Bologna, Italy. alessand.difrancesc3@unibo.it. Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin, 42, 40127, Bologna, Italy. alessand.difrancesc3@unibo.it. Plant Protection Department, Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000, Ljubljana, Slovenia. Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia. Center Agriculture Food Environment, University of Trento/Fondazione Edmund Mach, 38010, San Michele all'Adige, TN, Italy. Research and Innovation Centre, Fondazione Edmund Mach, via Mach 1, 38010, San Michele all' Adige, Trento, Italy. Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin, 42, 40127, Bologna, Italy. CRIOF-Department of Agricultural Sciences, University of Bologna, Via Gandolfi, 19, 40057, Cadriano, Bologna, Italy" |
| Journal Title: | World J Microbiol Biotechnol |
| DOI: | 10.1007/s11274-020-02947-7 |
| ISSN/ISBN: | 1573-0972 (Electronic) 0959-3993 (Print) 0959-3993 (Linking) |
| Abstract: | "Aureobasidium strains isolated from diverse unconventional environments belonging to the species A. pullulans, A. melanogenum, and A. subglaciale were evaluated for Volatile Organic Compounds (VOCs) production as a part of their modes of action against Botrytis cinerea of tomato and table grape. By in vitro assay, VOCs generated by the antagonists belonging to the species A. subglaciale showed the highest inhibition percentage of the pathogen mycelial growth (65.4%). In vivo tests were conducted with tomatoes and grapes artificially inoculated with B. cinerea conidial suspension, and exposed to VOCs emitted by the most efficient antagonists of each species (AP1, AM10, AS14) showing that VOCs of AP1 (A. pullulans) reduced the incidence by 67%, partially confirmed by the in vitro results. Conversely, on table grape, VOCs produced by all the strains did not control the fungal incidence but were only reducing the infection severity (< 44.4% by A. pullulans; < 30.5% by A. melanogenum, and A. subglaciale). Solid-phase microextraction (SPME) and subsequent gas chromatography coupled to mass spectrometry identified ethanol, 3-methyl-1-butanol, 2-methyl-1-propanol as the most produced VOCs. However, there were differences in the amounts of produced VOCs as well as in their repertoire. The EC(50) values of VOCs for reduction of mycelial growth of B. cinerea uncovered 3-methyl-1-butanol as the most effective compound. The study demonstrated that the production and the efficacy of VOCs by Aureobasidium could be directly related to the specific species and pathosystem and uncovers new possibilities for searching more efficient VOCs producing strains in unconventional habitats other than plants" |
| Keywords: | Aureobasidium/*chemistry Botrytis/*drug effects Butanols/isolation & purification/pharmacology Gas Chromatography-Mass Spectrometry Solanum lycopersicum/*growth & development/microbiology Microbial Sensitivity Tests Mycelium/drug effects Pentanols/isolati; |
| Notes: | "MedlineDi Francesco, A Zajc, J Gunde-Cimerman, N Aprea, E Gasperi, F Placi, N Caruso, F Baraldi, E eng Germany 2020/10/18 World J Microbiol Biotechnol. 2020 Oct 17; 36(11):171. doi: 10.1007/s11274-020-02947-7" |
