| Title: | Identification of a cell death pathway in Candida albicans during the response to pheromone |
| Author(s): | Alby K; Schaefer D; Sherwood RK; Jones SK; Bennett RJ; |
| Address: | "Department of Molecular Microbiology and Immunology, Brown University, Providence, RI 02912, USA" |
| ISSN/ISBN: | 1535-9786 (Electronic) 1535-9778 (Print) 1535-9786 (Linking) |
| Abstract: | "Mating in hemiascomycete yeasts involves the secretion of pheromones that induce sexual differentiation in cells of the opposite mating type. Studies in Saccharomyces cerevisiae have revealed that a subpopulation of cells experiences cell death during exposure to pheromone. In this work, we tested whether the phenomenon of pheromone-induced death (PID) also occurs in the opportunistic pathogen Candida albicans. Mating in C. albicans is uniquely regulated by white-opaque phenotypic switching; both cell types respond to pheromone, but only opaque cells undergo the morphological transition and cell conjugation. We show that approximately 20% of opaque cells, but not white cells, of laboratory strain SC5314 experience pheromone-induced death. Furthermore, analysis of mutant strains revealed that PID was significantly reduced in strains lacking Fig1 or Fus1 transmembrane proteins that are induced during the mating process and, we now show, are necessary for efficient mating in C. albicans. The level of PID was also Ca(2+) dependent, as chelation of Ca(2+) ions increased cell death to almost 50% of the population. However, in contrast to S. cerevisiae PID, pheromone-induced killing of C. albicans cells was largely independent of signaling via the Ca(2+)-dependent protein phosphatase calcineurin, even when combined with the loss of Cmk1 and Cmk2 proteins. Finally, we demonstrate that levels of PID vary widely between clinical isolates of C. albicans, with some strains experiencing close to 70% cell death. We discuss these findings in light of the role of prodeath and prosurvival pathways operating in yeast cells undergoing the morphological response to pheromone" |
| Keywords: | "Aspartic Acid Endopeptidases/genetics/physiology Base Sequence Calcium Signaling Candida albicans/*cytology/*drug effects/genetics/physiology Conjugation, Genetic DNA, Fungal/genetics Genes, Fungal HSP90 Heat-Shock Proteins/genetics/physiology Humans In V;" |
| Notes: | "MedlineAlby, Kevin Schaefer, Dana Sherwood, Racquel Kim Jones, Stephen K Jr Bennett, Richard J eng AI081704/AI/NIAID NIH HHS/ T32 GM007601/GM/NIGMS NIH HHS/ R21 AI081560/AI/NIAID NIH HHS/ AI081560/AI/NIAID NIH HHS/ F31DE019752/DE/NIDCR NIH HHS/ F31AI075607/AI/NIAID NIH HHS/ F31 AI075607/AI/NIAID NIH HHS/ F31 DE019752/DE/NIDCR NIH HHS/ R21AI081560/AI/NIAID NIH HHS/ R01 AI081704/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2010/09/28 Eukaryot Cell. 2010 Nov; 9(11):1690-701. doi: 10.1128/EC.00155-10. Epub 2010 Sep 24" |
